关键词: 补骨脂乙素, 舌肿瘤, 癌, 鳞状细胞, 细胞增殖, 细胞凋亡

Abstract:

Objective: To observe the effects of isobavachalcone (IBC) on the proliferation and apoptosis of tongue squamous cell carcinoma Tca-8113 cells,and to explore their mechanisms. Methods: The Tca8113 cells cultured in vitro were divided into control group and different doses (0,10,20,40,80 μmol·L^-1) of IBC groups. The inhibitory rates of cell proliferation were detected by MTT method.The apoptosis was detected by flow cytometry.Western blotting method was used to detect the expressions of Akt,p-Akt,Erk,p-Erk,Bax,Bcl-2 and Caspase-3 proteins in the Tca8113 cells in various groups. Results: The MTT results showed that the inhibitory rates of proliferation of Tca8113 cells were increased in a concentration- and time- dependent manner;the IC₅₀ at 12,24 and 48 h were (285.13±8.97),(132.40±7.76), and (58.56±5.93) μmol·L^-1, respectively;and there were significant differences between different time points (P < 0.05).The FCM results showed that the apoptotic rates of Tca8113 cells in 20 and 40 μmol·L^-1 IBC groups at 48 h were (8.21±2.32)% and (22.45±1.18)%, respectively;compared with control group (1.69%±0.65%),the differences were statistically significant (P < 0.05). The Western blotting results showed that the expression levels of Bcl-2,p-Akt, and p-Erk, in 20 and 40 μmol·L^-1 IBC groups were decreased and the expression levels of Bax were increased compared with control group (P < 0.05);the expression levels of Akt and Erk had no significant changes(P > 0.05).The expression levels of Caspase-3 in Tca8113 cells in 40 μmol·L^-1 IBC group at 48 h were increased compared with control group (P < 0.05). Conclusion: IBC could inhibit the proliferation and induce the apoptosis of Tca8113 cells;Akt and Erk signaling pathway may be the pathway of IBC to induce the apoptosis of tumor cells.

Key words: isobavachalcone, tongue neoplasms, carcinoma,squamous cell, cell proliferation, apoptosis