整联蛋白α亚基1基因单核苷酸多态性与非贲门胃癌发病风险的关联性分析

doi:10.13481/j.1671-587x.20190318

吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (03): 577-581.doi: 10.13481/j.1671-587x.20190318

• 临床研究 • 上一篇

整联蛋白α亚基1基因单核苷酸多态性与非贲门胃癌发病风险的关联性分析

高芳¹, 马立聪^2,3, 董文杰², 田旭阳³, 党彤³, 贾彦彬^2,3

1. 包头医学院医学技术学院微生物与免疫检验教研室, 内蒙古包头 014060;
2. 包头医学院基础医学与法医学院病原生物学教研室, 内蒙古包头 014060;
3. 包头医学院第二附属医院内蒙古消化病研究所, 内蒙古包头 014030

收稿日期:2018-09-19 发布日期:2019-06-05
通讯作者: 贾彦彬,教授,硕士研究生导师(Tel:0472-7167832,E-mail:jyb690318@hotmail.com);党彤,教授,硕士研究生导师(Tel:0472-3169902,E-mail:dtong999@sina.com) E-mail:jyb690318@hotmail.com;dtong999@sina.com
作者简介:高芳(1982-),女,内蒙古自治区包头市人,讲师,医学硕士,主要从事肿瘤分子生物学方面的研究。
基金资助:
国家自然科学基金资助课题（81250024，81650017）；内蒙古自治区自然科学基金资助课题（2016MS0805）；内蒙古自治区高等学校科研项目资助课题（NJZY204）；包头医学院科学研究基金资助课题（BYJJ-DF 201603）

Analysis on association between single nucleotide polymorphisms of ITGA1 gene and risk of non-cardia gastric cancer

GAO Fang¹, MA Licong^2,3, DONG Wenjie², TIAN Xuyang³, DANG Tong³, JIA Yanbin^2,3

1. Department of Microbiology and Immunology Laboratory, School of Medical Technology, Baotou Medical College, Baotou 014060, China;
2. Department of Pathogenic Biology, School of Basic Medicine and Forensic Medicine, Baotou Medical College, Baotou 014060, China;
3. Inner Mongolia Institute of Digestive Diseases, Second Affiliated Hospital, Baotou Medical College, Baotou 014030, China

Received:2018-09-19 Published:2019-06-05

摘要/Abstract

摘要： 目的：探讨整联蛋白α亚基1（ITGA1）基因单核苷酸多态性（SNP）与非贲门胃癌发病的关联性，阐明ITGA1基因多态性在非贲门胃癌发病中的作用。方法：采用病例-对照研究设计，在内蒙古自治区包头市汉族人群中选取288例非贲门胃癌患者为病例组，社区体检281人为对照组。采用TaqMan方法对2组研究对象ITGA1基因rs1862610、rs2432143和rs2447867位点的SNP进行基因分型，采用Haploview 4.0软件构建单体型，并采用非条件性Logistic回归计算比值比（OR）及其95%可信区间（95％CI），以评估各等位基因和基因型频数分布及单体型与非贲门胃癌发病风险的关联性。结果：2组研究对象ITGA1基因rs1862610、rs2432143和rs2447867位点的SNP与非贲门胃癌发病风险无关联（P>0.05）；rs1862610和rs2432143位点的SNP存在强连锁不平衡（D’=1），构成单体型块，与携带单体型CT者比较，携带单体型CC者非贲门胃癌的发病风险升高（CC vs CT：OR=1.42，95% CI：1.02~1.99）。结论：在内蒙古自治区包头市汉族人群中ITGA1基因多态性可能在非贲门胃癌的发病中起一定作用，携带SNP rs1862610-rs2432143 CC单体型者非贲门胃癌的发病风险升高。

关键词: 整联蛋白α亚基1, 基因多态性, 胃肿瘤, 遗传易感性

Abstract: Objective:To investigate the associations between single nucleotide polymorphisms (SNP) of integrin α-subunit 1 (ITGA1) gene and the risk of non-cardia gastric cancer, and to elucidate the roles of ITGA1 gene polymorphisms in the risk of non-cardia gastric cancer. Methods:The case-control study design was used. A total of 288 patients with non-cardia gastric cancer were selected as case group, and 281 community physical examinees were selected as control group in Han population in Boatou City in Inner Mongolia Autonomous Region. The SNP of rs1862610, rs2432143, and rs2447867 sites of subjects in two groups were genotyped by using TaqMan method,and the haplotype was constructed by Haploview software 4.0. The odds ratio (OR) and 95% confidence intervals (95%CI) were measured by conditional Logistic regression analysis to evaluate the correlations between the frequencies of allele, genotype, and haplotypes and the susceptibility to non-cardia gastric cancer. Results:The SNP of rs1862610, rs2432143, and rs2447867 sites in ITGA1 gene were not associated with the risk of non-cardia gastric cancer (P>0.05). The SNP of rs1862610 and rs2432143 sites were in strong linkage disequilibrium(D'=1) and formed a block. Compared with those who carried haplotype CT, those who carried haplotype CC had an increasing risk of non-cardiac gastric cancer(CC vs CT:OR=1.42, 95% CI:1.02-1.99). Conclusion:The ITGA1 gene polymorphisms may play a role in the risk of non-cardia gastric cancer in Han population in Baotou in Inner Mongolia Autonomous Region. The risk of non-cardia gastric cancer in the person who carries the SNP rs1862610-rs2432143 CC haplotype is increased.

Key words: integrin α-subunit 1, polymorphism, stomach neoplasms, susceptibility

中图分类号:

R394.5

高芳, 马立聪, 董文杰, 田旭阳, 党彤, 贾彦彬. 整联蛋白α亚基1基因单核苷酸多态性与非贲门胃癌发病风险的关联性分析[J]. 吉林大学学报(医学版), 2019, 45(03): 577-581.

GAO Fang, MA Licong, DONG Wenjie, TIAN Xuyang, DANG Tong, JIA Yanbin. Analysis on association between single nucleotide polymorphisms of ITGA1 gene and risk of non-cardia gastric cancer[J]. Journal of Jilin University(Medicine Edition), 2019, 45(03): 577-581.

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整联蛋白α亚基1基因单核苷酸多态性与非贲门胃癌发病风险的关联性分析

Analysis on association between single nucleotide polymorphisms of ITGA1 gene and risk of non-cardia gastric cancer

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