神经生长因子对脑梗死大鼠脑组织中生长分化因子15表达水平的影响

doi:10.13481/j.1671-587x.20190317

摘要/Abstract

摘要： 目的：探讨神经生长因子（NGF）对脑梗死大鼠脑组织中生长分化因子15（GDF-15）表达水平的影响，阐明NGF对脑梗死大鼠的作用机制。方法：采用颈内动脉线栓法制备大脑中动脉闭塞（MCAO）大鼠模型。将54只大鼠随机分为假手术组、模型组和NGF组，每组18只。NGF组大鼠腹腔注射50 μg·kg^-1 NGF，假手术组（仅分离动脉，不结扎栓线）和模型组大鼠腹腔注射等体积生理盐水。于造模后1、3和7 d进行神经功能评分，HE染色法观察各组大鼠脑组织神经元病理形态表现，免疫组织化学染色检测各组大鼠脑组织中GDF-15阳性细胞数，ELISA法检测各组大鼠脑组织中GDF-15表达水平。结果：HE染色，造模后7 d NGF组大鼠神经细胞坏死程度较低，细胞间质水肿、胶质细胞增生程度均较轻。与假手术组比较，模型组和NGF组大鼠造模后1、3和7 d的神经功能评分明显升高（P<0.05），GDF-15阳性细胞数明显升高（P<0.05），脑组织中GDF-15表达水平均明显升高（P<0.05）。与模型组比较，造模后3和7 d时NGF组大鼠神经功能评分降低（P<0.05），各时间点GDF-15阳性细胞数均明显升高（P<0.05），各时间点脑组织中GDF-15表达水平明显升高（P<0.05）。结论：NGF可上调大鼠脑组织中GDF-15表达水平，改善神经功能，达到保护脑神经的作用。

关键词: 脑梗死, 神经生长因子, 生长分化因子15, 神经功能, 大鼠,SD

Abstract: Objective:To investigate the effect of nerve growth factor (NGF) on the expression level of growth differentiation factor-15(GDF-15) in the brain tissue of the rats with cerebral infarction, and to elucidate the mechanism of NGF in the rats with cerebral infarction. Methods:The middle cerebral artery occlusion (MCAO) models were established by the Longa's method. A total of 54 SD rats were randomly divided into sham operation group, model group and NGF group, and there were 18 rats in each group.The rats in NGF group were given NGF (50 μg·kg^-1) by intraperitoneal injection,and equal volume of normal saline was given to the rats in sham operation (the artery was isolated without ligation) group and model group. The neurological function scores of the rats in various groups were measured 1, 3 and 7 d after operation; HE staining was used to observe the pathomorphology of nerves in brain tissue and immunohistochemical staining was used to detect the number of GDF-15 positive cells; the expression levels of GDF-15 in brain tissue of the rats in various groups were detected by ELISA method. Results:The results of HE staining showed that the degrees nerve cell necrosis, interstitial edema and glial cell proliferation were relatively low in NGF group 7 d after operation. Compared with sham operation group, the neurological function scores, the number of GDF-15 positive cells,and the expression levels of GDF-15 in brain tissue of the rats in model group and NGF group were significantly increased (P<0.05). Compared with model group, the neurological function scores of the rats in NGF group were significantly decreased 3 and 7 d after operation (P<0.05), and the number of GDF-15 positive cells and the expression levels of GDF-15 in brain tissue of the rats in NGF group were significantly increased at different time points after operation (P<0.05). Conclusion:NGF can protect the brain nerve by up-regulating the expression level of GDF-15 in brain tissue and improving the nerve function.

Key words: cerebral infarction, nerve growth factor, growth differentiation factor 15, neurological function, rats,SD

中图分类号:

R743.33

焦光美, 单海雷, 窦志杰, 赵亮, 张晓璇, 康玲伶, 马征, 杨宁. 神经生长因子对脑梗死大鼠脑组织中生长分化因子15表达水平的影响[J]. 吉林大学学报(医学版), 2019, 45(03): 572-576.

JIAO Guangmei, SHAN Hailei, DOU Zhijie, ZHAO Liang, ZHANG Xiaoxuan, KANG Lingling, MA Zheng, YANG Ning. Effect of nerve growth factor on expression level of growth differentiation factor-15 in brain tissue of rats with cerebral infarction[J]. Journal of Jilin University(Medicine Edition), 2019, 45(03): 572-576.

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