YAP对非小细胞肺癌细胞增殖和迁移能力的影响

doi:10.13481/j.1671-587x.20190622

吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (06): 1320-1326.doi: 10.13481/j.1671-587x.20190622

YAP对非小细胞肺癌细胞增殖和迁移能力的影响

周宁¹, 郭纪伟², 代娟娟², 李雪琳², 席思川², 宫凯凯², 武艳²

1. 滨州医学院附属医院耳鼻咽喉头颈外科, 山东滨州 256600;
2. 滨州医学院附属医院肿瘤研究中心, 山东滨州 256600

收稿日期:2019-01-17 出版日期:2019-12-05 发布日期:2019-12-05
通讯作者: 武艳,讲师(Tel:0543-3258276,E-mail:wuyan55@126.com);宫凯凯,讲师(Tel:0543-3258276,E-mail:gongkaikai1005@163.com) E-mail:wuyan55@126.com;gongkaikai1005@163.com
作者简介:周宁(1986-),男,山东省滨州市人,医师,医学硕士,主要从事肿瘤发病机制方面的研究。
基金资助:
山东省科技厅重点研发计划项目资助课题（2019GSF107099）；山东省卫计委医药卫生科技发展计划项目资助课题（2017WS154）；山东省科技厅自然科学基金资助课题（ZR2016HM57，ZR2017PH028，ZR2018BB024）

Effect of YAP on proliferation and migration of non-small cell lung cancer cells

ZHOU Ning¹, GUO Jiwei², DAI Juanjuan², LI Xuelin², XI Sichuan², GONG Kaikai², WU Yan²

1. Department of Otolaryngologyand Head-Neck Surgery, Affiliated Hospital, Binzhou Medical University, Binzhou 256603, China;
2. Cancer Research Center, Affiliated Hospital, Binzhou Medical University, Binzhou 256603, China

Received:2019-01-17 Online:2019-12-05 Published:2019-12-05

摘要/Abstract

摘要： 目的：探讨长链非编码RNA （lncRNA） MALAT1在YAP调控的非小细胞肺癌（NSCLC）细胞增殖和迁移中的作用，并阐明其作用机制。方法：选择A549和H1299细胞系分为Scramble siRNA组（转染Scramble siRNA）、siYAP-1组（转染siYAP-1）和siYAP-2组（转染siYAP-2）。RT-PCR和Western blotting法检测各组细胞中YAP和MALAT1mRNA及YAP蛋白表达水平；在A549和H1299细胞中分别转染pcDNA3.1-YAP质粒（pcDNA3.1-YAP组）、共转染pcDNA3.1-YAP质粒和siMALAT1-2（pcDNA3.1-YAP+siMALAT1-2组）及对照质粒（pcDNA3.1-YAP+Scramble siRNA组），CCK-8法检测各组细胞增殖能力，细胞划痕实验检测各组细胞迁移能力。结果：siYAP-1组和siYAP-2组A549和H1299细胞中YAP mRNA及蛋白表达水平均明显低于Scramble siRNA组（P<0.05），siYAP-1组和siYAP-2组A549和H1299细胞中MALAT1mRNA表达水平低于Scramble siRNA组（P<0.05）；与pcDNA3.1-YAP组比较，pcDNA3.1-YAP+siMALAT1-2组A549和H1299细胞增殖能力降低（P<0.05），H1299细胞迁移能力降低（P<0.05）。结论：在NSCLC细胞中，YAP通过调控lncRNA MALAT1的表达促进细胞的增殖和迁移。

关键词: YAP, MALAT1, 肺肿瘤, 细胞增殖, 迁移

Abstract: Objective: To investigate the role of long non-coding RNA(lncRNA) MALAT1 in the proliferation and migration of YAP-mediated non-small cell lung cancer (NSCLC) cells,and to elucidate its mechanism. Methods: The A549 and H1299 cell lines were selected and divided into Scramble siRNA group(transfected with Scramble siRNA),siYAP-1 group(transfected with siYAP-1)and siYAP-2 group(transfected with siYAP-2). RT-PCR and Western blotting methods were used to detect the expression levels of YAP and MALAT1 mRNA and YAP protein in the cells in variouis groups. The pcDNA3.1-YAP plasmid(pcDNA3.1-YAP group), pcDNA3.1-YAP plasmid plus siMALAT1-2 (pcDNA3.1-YAP+siMALAT1-2 group)and the control plasmid (pcDNA3.1-YAP+Scramble siRNA group)were transfected into the A549 and H1299 cells, respectively.CCK-8 method and scratch assay were used to detect the proliferation and migration abilities of the cells in various groups. Results: The expression levels of YAP mRNA and protein in the A549 and H1299 cells in siYAP-1 and siYAP-2 groups were significantly lower than those in Scramble siRNA group(P<0.05), and the expression levels of MALAT1 mRNA in the cells in siYAP-1 and siYAP-2 groups were significantly lower than those in Scramble siRNA group (P<0.05).Compared with pcDNA3.1-YAP group, the proliferation abilities of the A549 and H1299 cells in pcDNA3.1-YAP+siMALAT1-2 group were decreased(P<0.05),the migration ability of the H1299 cells in pcDNA3.1-YAP+siMALAT1-2 group was decreased(P<0.05). Conclusion: In the NSCLC cells, YAP promotes the cell proliferation and migration by regulating the expression of lncRNA MALAT1.

Key words: YAP, MALAT1, lung neoplasms, cell proliferation, migration

中图分类号:

R734.2

周宁, 郭纪伟, 代娟娟, 李雪琳, 席思川, 宫凯凯, 武艳. YAP对非小细胞肺癌细胞增殖和迁移能力的影响[J]. 吉林大学学报(医学版), 2019, 45(06): 1320-1326.

ZHOU Ning, GUO Jiwei, DAI Juanjuan, LI Xuelin, XI Sichuan, GONG Kaikai, WU Yan. Effect of YAP on proliferation and migration of non-small cell lung cancer cells[J]. Journal of Jilin University(Medicine Edition), 2019, 45(06): 1320-1326.

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YAP对非小细胞肺癌细胞增殖和迁移能力的影响

Effect of YAP on proliferation and migration of non-small cell lung cancer cells

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