复方苦参注射液对鼻咽癌CNE-2细胞的放射增敏作用

doi:10.13481/j.1671-587x.20190632

吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (06): 1384-1388.doi: 10.13481/j.1671-587x.20190632

复方苦参注射液对鼻咽癌CNE-2细胞的放射增敏作用

郑剑霄¹, 詹文婷¹, 周晓春¹, 孔怡琳¹, 高蕾¹, 李工¹, 王苏美²

1. 广州中医药大学第二附属医院放疗科广东省中医院放疗科, 广东广州 510120;
2. 广州中医药大学第二附属医院肿瘤科广东省中医院肿瘤科, 广东广州 510120

收稿日期:2019-09-01 出版日期:2019-12-05 发布日期:2019-12-05
通讯作者: 王苏美,副研究员(Tel:020-39318473,E-mail:wangsumei198708@163.com) E-mail:wangsumei198708@163.com
作者简介:郑剑霄(1973-),男,江西省上饶县人,副主任医师,医学硕士,主要从事肿瘤放射治疗方面的研究。
基金资助:
广东省卫计委医学科研基金资助课题（A2018251）

Radiosensitization of Compound Sophora Flavescens Injection on nasopharyngeal carcinoma CNE-2 cells

ZHENG Jianxiao¹, ZHAN Wenting¹, ZHOU Xiaochun¹, KONG Yilin¹, GAO Lei¹, LI Gong¹, WANG Sumei²

1. Department of Radiotherapy, Sencond Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, China;
2. Department of Oncology, Sencond Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, China

Received:2019-09-01 Online:2019-12-05 Published:2019-12-05

摘要/Abstract

摘要： 目的：观察复方苦参注射液对人鼻咽癌CNE-2细胞放射敏感性的影响，并探讨其作用机制。方法：常规体外培养人鼻咽癌CNE-2细胞，采用四甲基偶氮唑盐（MTT）法检测不同浓度复方苦参注射液分别处理CNE-2细胞24、48和72 h后时细胞增殖抑制率，计算复方苦参注射液对CNE-2细胞的半数抑制浓度（IC₅₀）。CNE-2细胞分为对照组（不照射、不给药）、放射组（给予剂量为2和4 GyX射线照射）和联合组（给予不同浓度复方苦参注射液处理后再接受X射线照射）。MTT法检测各组CNE-2细胞增殖抑制率，流式细胞术检测各组CNE-2细胞凋亡率，克隆形成试验检测各组CNE-2细胞存活率。结果：不同浓度复方苦参注射液处理CNE-2细胞24、48和72 h后，细胞增殖抑制率较未经复方苦参注射液处理的CNE-2细胞升高（P<0.05），且呈剂量依赖性；复方苦参注射液处理48 h时细胞增殖抑制率高于24 h时（P<0.05）。放射组CNE-2细胞凋亡率高于对照组（P<0.05），联合组CNE-2细胞凋亡率较放射组升高（P<0.05）；联合组CNE-2细胞存活率低于放射组（P<0.05）。结论：复方苦参注射液能促进放射线诱导的鼻咽癌CNE-2细胞凋亡，从而对CNE-2细胞具有放射增敏作用。

关键词: 复方苦参注射液, 细胞凋亡, 放射增敏, 鼻咽肿瘤

Abstract: Objective: To observe the effect of Compound Sophora Flavescens Injection on the radiosensitivity of the human nasopharyngeal carcinoma CNE-2 cells,and to explore its mechanism. Methods: The human nasopharyngeal carcinoma CNE-2 cells were cultured in vitro. The inhibitory rates of proliferation of CNE-2 cells after treated with different concentrations of Compound Sophora Flavescens Injection for 24, 48, and 72 h were detected by MTT method. The semi-inhibitory concentration (IC₅₀) of Compound Sophora Flavescens Injection on CNE-2 cells was calculated. The CNE-2 cells were divided into control group(without irradiation and adminnistration), irradiation group (treated with 2 and 4 Gy X-ray irradiation) and combination group(treated with different concentrations of Compound Sophora Flavescens Injection and then received X-ray irradiation). MTT assay was used to detect the inhibitory rates of proliferation of the CNE-2 cells in various groups, flow cytometry was used to detect the apoptotic rates of the CNE-2 cells in various groups,and clone formation test was used to detect the survival rates of the CNE-2 cells in various groups. Results: After treated with different concentrations of Compound Kushen Injection for 24, 48 and 72 h, the inhibitory rates of proliferation of the CNE-2 cells were increased compared with the CNE-2 cells without Compound Kushen Injection treatment (P<0.05) in an dose-dependent manner;the inhibitory rate of proliferation of the CNE-2 cells after treated with Compound Kushen injection for 48 h was higher than 24 h (P<0.05).The apoptotic rate of the CNE-2 cells in radiation group was higher than that in control group (P<0.05); the apoptotic rate of the CNE-2 cells in combination group was higher than that in radiation group (P<0.05);the survival rate of the CNE-2 cells in combination group was lower than that in radiation group(P<0.05). Conclusion: Compound Sophora Flavescens Injection can promote the irradiation-induced apoptosis of nasopharyngeal carcinoma CNE-2 cells, therefore, Compound Sophora Flavescens Injection has the radiosensitization effect on the CNE-2 cells.

Key words: Compound Sophora Flavescens Injection, apoptosis, radiosensitization, nasopharyngeal neoplasms

中图分类号:

R739.63

郑剑霄, 詹文婷, 周晓春, 孔怡琳, 高蕾, 李工, 王苏美. 复方苦参注射液对鼻咽癌CNE-2细胞的放射增敏作用[J]. 吉林大学学报(医学版), 2019, 45(06): 1384-1388.

ZHENG Jianxiao, ZHAN Wenting, ZHOU Xiaochun, KONG Yilin, GAO Lei, LI Gong, WANG Sumei. Radiosensitization of Compound Sophora Flavescens Injection on nasopharyngeal carcinoma CNE-2 cells[J]. Journal of Jilin University(Medicine Edition), 2019, 45(06): 1384-1388.

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复方苦参注射液对鼻咽癌CNE-2细胞的放射增敏作用

Radiosensitization of Compound Sophora Flavescens Injection on nasopharyngeal carcinoma CNE-2 cells

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