M1型巨噬细胞相关因子在重度慢性牙周炎患者牙龈组织中的表达及其意义

doi:10.13481/j.1671-587x.20200227

吉林大学学报(医学版) ›› 2020, Vol. 46 ›› Issue (02): 372-376.doi: 10.13481/j.1671-587x.20200227

• 临床研究 • 上一篇

M1型巨噬细胞相关因子在重度慢性牙周炎患者牙龈组织中的表达及其意义

蔡如佳¹, 关宁², 刘乙臻¹, 高秀秋¹, 王琳源¹

1. 锦州医科大学附属第二医院牙周黏膜科, 辽宁锦州 121000;
2. 锦州医科大学附属第一医院脑与脊髓损伤重点实验室, 辽宁锦州 121000

收稿日期:2019-04-22 发布日期:2020-04-07
通讯作者: 王琳源,副主任医师,硕士研究生导师(Tel:0416-2655132,E-mail:wanglinyuan2006@163.com) E-mail:wanglinyuan2006@163.com
作者简介:蔡如佳(1993-),女,辽宁省凤城市人,在读医学硕士,主要从事牙周炎免疫调节方面的研究。
基金资助:
国家自然科学基金青年基金资助课题（81600870）；辽宁省博士启动基金资助课题（201601360）

Expressions of M1 macrophage-related factors in gingiva tissue of patients with severe chronic periodontitis and their significances

CAI Rujia¹, GUAN Ning², LIU Yizhen¹, GAO Xiuqiu¹, WANG Linyuan¹

1. Department of Periodontics and Mucosa, Second Affiliated Hospital, Jinzhou Medical University, Jinzhou 121000, China;
2. Key Laboratory of Brain and Spinal Cord Injury, First Affiliated Hospital, Jinzhou Medical University, Jinzhou 121000, China

Received:2019-04-22 Published:2020-04-07

摘要/Abstract

摘要： 目的：探讨M1型巨噬细胞相关因子诱导型一氧化氮合酶（iNOS）和信号传导及转录激活因子1（STAT1）在重度慢性牙周炎患者牙龈组织中的表达情况，阐明M1型巨噬细胞在慢性牙周炎发生发展中的可能作用。方法：选择15例重度慢性牙周炎患者的病损牙龈组织作为实验组，15例需要拔除第三磨牙患者的健康牙龈组织作为对照组。采用RT-PCR法检测2组患者牙龈组织中iNOS和STAT1 mRNA表达水平，Western blotting法检测2组患者牙龈组织中iNOS和STAT1蛋白表达水平。结果：与对照组比较，实验组患者牙龈组织中iNOS和STAT1 mRNA表达水平明显升高（P<0.01）；与对照组比较，实验组患者牙龈组织中iNOS和STAT1蛋白表达水平明显升高（P<0.01）。结论：在重度慢性牙周炎中M1型巨噬细胞介导的免疫应答增强，表明M1型巨噬细胞参与牙周组织的炎症反应和组织损伤。

关键词: M1型巨噬细胞, 慢性牙周炎, 诱导型一氧化氮合酶, 信号传导及转录激活因子1

Abstract: Objective: To investigate the expressions of M1 macrophage-related factors induced nitric oxide synthase(iNOS) and signal transducers and activators of transcription 1(STAT1) in gingiva tissue of the patients with severe chronic periodontitis, and to elucidate the possible role of M1 macrophages in the occurrence and development of chronic periodontitis. Methods: The gingiva tissue of 15 patients with severe chronic periodontitis was selected as experimental group, and 15 cases of healthy gingiva tissue of the patients requiring removal of the third molars were used as control group.The expression levels of iNOS and STAT1 mRNA in the gingiva tissues of the patients in two groups were detected by RT-PCR.The expression levels of iNOS and STAT1 proteins in the gingiva tissues of the patients in two groups were detected by Western blotting method. Results: Compared with control group,the expression levels of iNOS and STAT1 mRNA in the gingiva tissue of the patients in experimental group were significantly increased (P<0.01);compared with control group,the expression levels of iNOS and STAT1 proteins in the gingiva tissue of the patients in experiment group were also significantly increased (P<0.01). Conclusion: M1 macrophage-mediated immune response is enhanced in severe chronic periodontitis, suggesting that M1 macrophages are involved in the inflammatory response and tissue damage of periodontal tissue.

Key words: M1 macrophage, chronic periodontitis, induced nitric oxide synthase, signal transducers and activators of transcription 1

中图分类号:

R781.42

蔡如佳, 关宁, 刘乙臻, 高秀秋, 王琳源. M1型巨噬细胞相关因子在重度慢性牙周炎患者牙龈组织中的表达及其意义[J]. 吉林大学学报(医学版), 2020, 46(02): 372-376.

CAI Rujia, GUAN Ning, LIU Yizhen, GAO Xiuqiu, WANG Linyuan. Expressions of M1 macrophage-related factors in gingiva tissue of patients with severe chronic periodontitis and their significances[J]. Journal of Jilin University(Medicine Edition), 2020, 46(02): 372-376.

参考文献

[1] XU R,ZENG G,WANG S,et al.Periodontitis promotes the diabetic development of obese rat via miR-147 induced classical macrophage activation[J].Biomed Pharmacother,2016,83:892-897.
[2] 王琳源,关宁,林晓萍.Treg细胞介导的免疫应答在牙周炎小鼠中的研究[J].实用口腔医学杂志,2015,31(3):318-322.
[3] 袁中政,刘引,申玉芹,等.牙周炎患者自身组织核酸对小鼠巨噬细胞中NLRP3 mRNA表达的影响及其意义[J].吉林大学学报(医学版),2019,45(1):23-27.
[4] CEKICI A,KANTARCI A,HASTURK H,et al.Inflammatory and immune pathways in the pathogenesis of periodontal disease[J].Periodontology, 2014,64(1):57-80.
[5] GORDON S, MARTINEZ F O. Alternative activation of macrophages:mechanism and functions[J]. Immunity, 2010, 32(5):593-604.
[6] SIMA C, GLOGAUER M. Macrophage subsets and osteoimmunology:tuning of the immunological recognition and effector systems that maintain alveolar bone[J]. Periodontology, 2013, 63(1):80-101.
[7] 梁红玲,陈斌,何莎莎,等.牙周炎和牙周健康状态的大鼠牙周组织中M1/M2型巨噬细胞的分布和比例[J].口腔疾病防治,2018,26(10):627-633.
[8] 中华口腔医学会牙周病学专业委员会.重度牙周炎诊断标准及特殊人群牙周病治疗原则的中国专家共识[J].中华口腔医学杂志,2017,52(2):67-71.
[9] STINGU C S,JENTSCH H,EICK S,et al. Microbial profile of patients with periodontitis compared with healthy subjects[J]. Quintessence Int, 2012,43(2):e23-e31.
[10] 潘佳慧,唐秋玲,李格格,等.巨噬细胞极化在牙龈卟啉单胞菌促进牙周炎发生发展中的作用[J].国际口腔医学杂志,2017,44(5):533-537.
[11] JAGANNATHAN R, LAVU V, RAO S R. Comparison of the proportion of non-classic (CD14+CD16+) monocytes/macrophages in peripheral blood and gingiva of healthy individuals and patients with chronic periodontitis[J].J Periodontol, 2014, 85(6):852-858.
[12] OPPERMANN R V, WEIDLICH P, MUSSKOPF M L. Periodontal disease and systemic complications[J]. Braz Oral Res, 2012,26:39-47.
[13] LAWRENCE T, NATOLI G. Transcriptional regulation of macrophage polarization:enabling diversity with identity[J]. Nat Rev Immunol, 2011, 11(11):750-761.
[14] WHYTE C S, BISHOP E T, RUCKERL D, et al. Suppressor of cytokine signaling (SOCS)1 is a key determinant of differential macrophage activation and function[J]. J Leukoc Biol, 2011, 90(5):845-854.
[15] MōGE J L, MEHRAJ V, CAPO C, et al. Macrophage polarization and bacterial infections[J]. Curr Opin Infect Dis, 2011, 24(3):230-234.
[16] GEMMELL E, MCHUGH G B, GRIECO D A, et al. Costimulatory molecules in human periodontal disease tissues[J]. J Periodont Res, 2001, 36(2):92-100.
[17] AALAEI-ANDABILI S H, REZAEI N.Toll-like receptor (TLR)-induced differential expression of microRNAs (MiRs) promotes proper immune response against infections:A systematic review[J]. J Infect, 2013, 67(4):251-264.
[18] HUSSAIN Q A, MCKAY I J, Gonzales-Marin C, et al. Detection of adrenomedullin and nitric oxide in different forms of periodontal disease[J]. J Periodont Res, 2016, 51(1):16-25.
[19] ADLER H, FRECH B, THONY M, et al. Inducible nitric oxide synthase in cattle. Differential cytokine regulation of nitric oxide synthase in bovine and murine macrophages[J]. J Immunol, 1995,154(9):4710-4718.
[20] HALL C J, BOYLE R H, ASTIN J W, et al. Immunoresponsive gene 1 augments bactericidal activity of macrophage-lineage cells by regulating β-oxidation-dependent mitochondrial ROS production[J]. Cell Metab, 2013, 18(2):265-278.
[21] MURRAY PJ. The JAK -STAT signaling pathway:input and output integration[J]. J Immunol, 2007, 178(5):2623-2629.
[22] HU J C, BROOKINGS W, ALDRIDGE M C. A case of solid pseudopapillary tumour of the pancreas and malignant mesothelioma[J]. J Gastrointest Cancer, 2007, 38(2/3/4):71-73.

M1型巨噬细胞相关因子在重度慢性牙周炎患者牙龈组织中的表达及其意义

Expressions of M1 macrophage-related factors in gingiva tissue of patients with severe chronic periodontitis and their significances

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

Metrics

本文评价

推荐阅读 0

[1]	许立硕, 黄玉, 金权, 刘晨光, 陈飞, 张莉, 马宁. 野菊花骨碎补复合中药制剂联合奥硝唑治疗慢性牙周炎的效果评价[J]. 吉林大学学报(医学版), 2019, 45(05): 1128-1133.
[2]	金权, 郑义, 许立硕, 黄玉, 马宁, 张莉. 骨膜蛋白在慢性牙周炎患者牙龈组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2019, 45(03): 634-638.
[3]	郑苔菁, 白远亮, 郑佳雯, 刘洪宏, 郭子君, 王萍. 克拉霉素和甲硝唑治疗慢性牙周炎疗效的Meta分析[J]. 吉林大学学报(医学版), 2019, 45(01): 130-136.
[4]	于洋, 张岳培, 王润泽, 刘师兵, 徐路, 徐冶. 氯化钴对人卵巢癌SKOV3细胞顺铂敏感性的影响[J]. 吉林大学学报(医学版), 2019, 45(01): 1-6.
[5]	陈飞, 史金先, 焦鹏, 金权, 许立硕, 张莉, 马宁. 牙周炎患者血清中内脂素和PGE2水平检测及其与牙周炎活动性的关系[J]. 吉林大学学报(医学版), 2018, 44(03): 563-567.
[6]	于艳, 韩亚琨, 张颖丽. 慢性牙周炎患者A型人格行为特征分布的调查分析[J]. 吉林大学学报(医学版), 2016, 42(02): 395-399.
[7]	任博，孙法威，张作凤，张宇新. 丹参酮ⅡA对帕金森病模型小鼠多巴胺能神经元的保护作用及其机制[J]. 吉林大学学报(医学版), 2014, 40(05): 947-952.
[8]	. 血必净对LPS诱导血管内皮细胞NO产生和iNOS及核转录因子κB蛋白表达的影响[J]. 吉林大学学报(医学版), 2014, 40(05): 997-1001.
[9]	辛维娜，蒋荣娜，石卓，盛辉. 甘草酸二铵对博莱霉素致肺纤维化大鼠血清iNOS及NO水平的影响及其抗肺纤维化的作用机制[J]. 吉林大学学报(医学版), 2013, 39(1): 38-41.
[10]	张田, 魏子峰,秦丽娟,周洪霞,张宇新. 罗格列酮对帕金森病模型小鼠中脑黑质iNOS和IL-6表达的抑制作用及其意义[J]. J4, 2012, 38(6): 1124-1128.
[11]	刘国利, 沈途, 张锦英. 异氟醚预处理对缺血再灌注损伤后幼兔心肌TNF-α和iNOS mRNA表达的影响及其机制[J]. J4, 2011, 37(4): 622-625.