关键词: 细胞分裂周期蛋白14B, 受精卵, 有丝分裂细胞周期, 小鼠

Abstract: Objective: To observe the expression and localization of cell division cycle 14B (CDC14B) in each cell cycle of the mouse one-cell fertilized eggs,and to elucidate preliminarily the mechanism of CDC14B regulating the early development of mammalian fertilized eggs. Methods: The superovulation technique was used to collect the mouse one-cell fertilized eggs. The expression levels of CDC14B mRNA and protein in the mouse one-cell stage fertilized eggs in different cell cycles were detected by RT-PCR and Western blotting methods, respectively. Co-localization of CDC14B and β-tubulin in the mouse one-cell fertilized eggs in different cell cycles was observed by cell immunofluorescence method. Results: Compared with G₁ phase, the expression levels of CDC14B mRNA and protein in the mouse one-cell fertilized eggs in S, G₂ and M phases were significantly increased(P<0.05 or P<0.01).The cellular immunofluorescence assay results showed that CDC14B (red fluorescence) and β-tubulin (green fluorescence) were co-located in the cortex of fertilized mouse eggs in G₁ phase and S phase,in the early G₂ phase, CDC14B and β-tubulin co-localized in the cortex and cytoplasm; in the late G₂ phase, part of CDC14B entered the nucleus; in the M phase, CDC14B and β-tubulin co-localized in the spindle. Conclusion: CDC14B dynamically expresses in each cell cycle of the mouse one-cell fertilized eggs. CDC14B has nuclear plasma spindle translocation, and the localization changes of CDC14B may regulate the spindle function.

Key words: cell division cycle 14B protein, fertilized eggs, mitosis cell cycle, mice