浆细胞样树突状细胞和Foxp3+调节性T细胞在结直肠癌组织中的表达及其意义

doi:10.13481/j.1671-587x.20200427

摘要/Abstract

摘要： 目的：探讨CD123⁺浆细胞样树突状细胞（pDCs）和叉状头转录因子3（Foxp3⁺）调节性T细胞（Tregs）在结直肠癌（CRC）组织中的表达及其在肿瘤引流淋巴结（TDLN）中的浸润，阐明其对CRC生物学行为的影响。方法：收集63例CRC手术切除病理标本、癌旁正常组织及相应TDLN，采用免疫组织化学方法检测不同组织中CD123⁺pDCs和Foxp3⁺Tregs的阳性细胞数，采用Spearman秩相关分析法检测二者表达相关性。结果：CRC患者癌组织中Foxp3⁺Tregs阳性细胞数高于癌旁正常组织（P<0.01）；淋巴结转移阳性患者癌组织中Foxp3⁺Tregs阳性细胞数高于癌旁正常组织（P<0.01）；转移肿瘤引流淋巴结（mTDLN）组织中Foxp3⁺Tregs阳性细胞数高于非转移肿瘤引流淋巴结（mfTDLN）（P<0.01）；TNM分期Ⅲ+Ⅳ期患者癌组织中Foxp3⁺Tregs阳性细胞数高于TNM分期Ⅰ+Ⅱ期患者（P<0.01）。CRC患者癌组织中CD123⁺pDCs阳性细胞数较低，但CD123阳性表达率明显高于癌旁正常组织（P<0.01）；mTDLN与mfTDLN组织中CD123⁺pDCs阳性细胞数比较差异无统计学意义（P>0.05）；mTDLN组织中pDCs与髓样树突状细胞（mDCs）的比值（pDCs/mDCs）明显高于mfTDLN组织（P<0.01）；pDCs/mDCs比值与Foxp3⁺Tregs阳性细胞数呈正相关关系（r=0.421，P<0.01）。结论：CRC的发生发展与癌组织局部微环境中CD123⁺pDCs阳性细胞数无关，与Foxp3⁺Tregs浸润数量有关。肿瘤组织中DCs亚群的变化与Foxp3⁺Tregs相关，其表达促进了CRC的发生发展。

关键词: 浆细胞样树突状细胞, 肿瘤微环境, 调节性T细胞, 结直肠肿瘤

Abstract: Objective: To investigate the expressions of CD123⁺ plasmacytoid dendritic cells(pDCs) and Foxp3⁺ regulatory cells(Tregs) in the colorectal cancer tissue (CRC) and their infiltrations in the tumor draining lymph nodes (TDLN), and to elucidate their effects on the biological behaviors of CRC. Methods: A total of 63 pathological specimens of removed CRC tissue, cancer adjacent normal mucosa tissue and TDLN were collected. The number of CD123⁺pDCs and Foxp3⁺Treg positive cells in different tissues were detected by immunohistochemical method. The correlation between the expressions of them were detected by Spearman analysis. Results: The number of Foxp3⁺Tregs positive cells in cancer tissue of the CRC patients was higher than that in adjacent normal mucosa tissue(P<0.01). The number of Foxp3⁺Tregs positive cells in cancer tissue of the patients with positive lymph node metastasis was higher than that in adjacent normal mucosa (P<0.01).The number of Foxp3⁺Tregs positive cells in metastatic TDLN (mTDLN) was higher than that in metastatic free TDLN (mfTDLN) (P<0.01).The number of Foxp3⁺Tregs psitive cells in cancer tissue in the patients in TNM Ⅲ+Ⅳstage was higher than that in the patients in TNM Ⅰ+Ⅱ stage (P<0.01).The number of CD123⁺pDCs positive cells in cancer tissue of the CRC patients was lower, and the positive expression rate of CD123 in CRC tissue was signifificantly higher than that in adjacent normal mucosa tissue (P<0.01).The number of CD123⁺pDCs positive cells in mfTDLN and mTDLN had no significant difference (P>0.05).The pDCs/myeloid dendritic cells(mDCs) ratio in mTDLN was signifificantly higher than that in mfTDLN(P<0.01).The correlative analysis results demonstrated that the ratio of pDCs/mDCs had a positive relationship with the number of Foxp3⁺ Tregs positive cells(r=0.421, P<0.01). Conclusion: The occurrence and development of CRC has nothing to do with the expression frequency of CD123⁺pDCs and is related to the amount of Foxp3⁺Tregs infiltration in the cancer tissue,The change of DCs subgroups in tumor tissue has a positive relationship with Foxp3⁺Tregs, which promotes the occurrence and development of CRC.

Key words: plasmacytoid dendritic cells, tumor microenvironment, regulatory T cells, colorectal neoplasms

中图分类号:

R735.3

王丹, 廖丹, 李红, 熊立秋, 武莹, 董营, 盖晓东. 浆细胞样树突状细胞和Foxp3⁺调节性T细胞在结直肠癌组织中的表达及其意义[J]. 吉林大学学报(医学版), 2020, 46(04): 834-838.

WANG Dan, LIAO Dan, LI Hong, XIONG Liqiu, WU Ying, DONG Ying, GAI Xiaodong. Expressions of plasmacytoid dendritic cells and Foxp3⁺ regulatory T cells in colorectal cancer tissue and their significances[J]. Journal of Jilin University(Medicine Edition), 2020, 46(04): 834-838.

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