人结直肠癌和肿瘤引流淋巴结组织中Foxp3+调节性T细胞和髓样树突状细胞的表达及其意义

doi:10.13481/j.1671-587x.20190325

摘要/Abstract

摘要： 目的：探讨Foxp3⁺调节性T细胞（Tregs）和CD11c⁺髓样树突状细胞（mDCs）在结直肠癌（CRC）组织中的表达及在肿瘤引流淋巴结（TDLN）组织中的浸润，并探讨其临床意义。方法：收集CRC患者手术切除的标本52例，采用免疫组织化学方法检测CRC患者标本及TDLN组织样本中Foxp3⁺ Tregs及CD11c⁺mDCs的表达，并探讨其表达频数与CRC患者临床病理特征的关系。结果：CRC患者癌组织中Foxp3⁺Tregs的阳性表达频数高于癌旁正常组织（P<0.01）；淋巴结转移阳性者癌组织中Foxp3⁺Tregs阳性表达频数高于淋巴结转移阴性者（P<0.01）；TNM分期Ⅲ+Ⅳ期组患者癌组织中Foxp3⁺Tregs阳性表达频数高于TNM分期Ⅰ+Ⅱ期组患者（P<0.01）；肿瘤引流阳性淋巴结（mTDLN）组织中Foxp3⁺ Tregs阳性表达频数明显高于肿瘤引流阴性淋巴结（mfTDLN）组织（P<0.01）。CRC患者癌组织中CD11c⁺ mDCs的阳性表达频数高于癌旁正常组织（P<0.01）；淋巴结转移阳性者癌组织中CD11c⁺ mDCs阳性表达频数低于淋巴结转移阴性者（P<0.01）；TNM分期Ⅲ+Ⅳ期组患者癌组织中CD11c⁺ mDCs阳性表达频数低于TNM分期Ⅰ+Ⅱ期组患者（P<0.01）；不同浸润深度癌组织中CD11c⁺ mDCs阳性表达频数比较差异有统计学意义（P<0.05）；mTDLN组织中CD11c⁺ mDCs阳性表达频数明显低于mfTDLN组织（P<0.01）。CRC和TDLN组织中Foxp3⁺ Tregs表达与CD11c⁺mDCs的表达无相关性（P>0.05）。结论：CRC的发生发展与癌组织局部微环境中Foxp3⁺ Tregs及CD11c⁺ mDCs的表达频数有关。Foxp3⁺ Tregs和CD11c⁺mDCs在抑制肿瘤微环境的细胞免疫中发挥作用。

关键词: 结直肠肿瘤, 肿瘤引流淋巴结, 肿瘤转移, 调节性T细胞, 髓样树突状细胞

Abstract: Objective:To explore the expressions of Foxp3⁺ Tregs and CD11c⁺ mDCs in the colorectal cancer (CRC) tissue and their infiltrations in the tumor draining lymph node (TDLN) tissue,and to explore the clinical significances. Methods:Fifty-two samples of surgical resection of the CRC patients were collected. The expressions of Foxp3⁺ Tregs and CD11c⁺ mDCs in the specimens of the CRC patients and TDLN tissue were detected by immunohistochemistry, and the relationship between the expression frequencies of Foxp3⁺ Tregs and CD11c⁺ mDCs and the clinicopathological characteristics of the patients were discussed. Results:The positive expression frequency of Foxp3⁺ Tregs in cancer tissue of the CRC patients was higher than that in adjacent normal mucosa tissue(P<0.01); The expression frequency of Foxp3⁺ Tregs in cancer tissue in the patients with positive lymph node metastasis was higher than that in the patients with negative lymph node metastasis(P<0.01); the expression frequency of Foxp3⁺ Tregs in the patients in TNM stage Ⅲ+Ⅳ was higher than that in TNM stage Ⅰ+Ⅱ(P<0.01); the positive expression frequency of Foxp3⁺ Tregs in metastatic TDLN (mTDLN) tissue was higher than that in metastatic free TDLN (mfTDLN) tissue(P<0.01). The positive expression frequency of CD11c⁺ mDCs in cancer tissue of the CRC patients was higher than that in adjacent normal mucosa tissue(P<0.01); the positive expression frequency of CD11c⁺ mDCs in the patients with positive lymph node metastasis was lower than that in the patients with negative lymph node metastasis(P<0.01);the positive expression frequency of CD11c⁺ mDCs in the patients in TNM stage Ⅲ+Ⅳ was lower than that in the patients in TNM stage Ⅰ+Ⅱ(P<0.01); the positive expression frequencies of CD11c⁺ mDCs in cancer tissue with different depths invasion were significantly different (P<0.05);the positive expression frequency of CD11c⁺ mDCs in mTDLN tissue was lower than that in mfTDLN tissue (P<0.01).There were no significant correlations between the expressions of Foxp3⁺ Tregs and CD11c⁺ mDCs in the CRC and TDLN tissues(P>0.05). Conclusion:The occurrence and development of CRC are related to the changes of Foxp3⁺ Tregs and CD11c⁺ mDCs expression frequencies in the local microenvironment of cancer tissue.Foxp3⁺ Tregs and CD11c⁺ mDCs play a role in inhibiting the cellular immunity in tumor microenvironment.

Key words: colorectal neoplasms, tumor draining lymph nodes, tumor metastasis, regulatory T cells, myeloid dentritic cells

中图分类号:

R735.35

王丹, 宋紫绮, 李怡飞, 历春, 董志恒, 董营, 盖晓东. 人结直肠癌和肿瘤引流淋巴结组织中Foxp3⁺调节性T细胞和髓样树突状细胞的表达及其意义[J]. 吉林大学学报(医学版), 2019, 45(03): 621-626.

WANG Dan, SONG Ziqi, LI Yifei, LI Chun, DONG Zhiheng, DONG Ying, GAI Xiaodong. Expressions of Foxp3⁺ regulatory T cells and myeloid dentritic cells in human colorectal cancer and tumor draining lymph node tissues and their significances[J]. Journal of Jilin University(Medicine Edition), 2019, 45(03): 621-626.

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