吉林大学学报(医学版) ›› 2013, Vol. 39 ›› Issue (3): 488-493.doi: 10.7694/jldxyxb20130313

• 基础研究 • 上一篇    下一篇

结肠安栓联合舒血宁对溃疡性结肠炎大鼠的治疗作用及其机制

李 辉1,赵丽晶2,杜 宇1,杨铁铮1,李国峰1,陈亮1,支晨阳1,李 娜3,周建华1   

  1. 1.长春中医药大学第一附属医院肛肠外科,吉林 长春 130117;2. 吉林大学白求恩医学院病理生理学教研室,吉林 长春 130021;3.长春中医药大学研发中心中药药理实验室,吉林 长春 130117
  • 收稿日期:2012-01-21 出版日期:2013-05-28 发布日期:2013-07-01
  • 通讯作者: 周建华(Tel:0431-86177301,E-mail:zjh5804@163.com) E-mail:zjh5804@163.com
  • 作者简介:李 辉(1978-),男,吉林省长春市人,医师,医学硕士,主要从事中医肛肠疾病的研究。
  • 基金资助:

    吉林省科技厅科研基金资助课题(20090923)

Therapeutic effect of suppository of Jiechang An combined with 
Shuxue Ning on rats with ulcerative colitis and their mechanisms

LI Hui1,ZHAO Li-jing2,DU Yu1,YANG Tie-zheng1,LI Guo-feng1,CHEN Liang1,ZHI Chen-yang1,LI Na3,ZHOU Jian-hua1   

  1. 1.Department of Anal-Colorectal Surgery,First Affiliated Hospital,Changchun University of Chinese Medicine,Changchun 130117,China;2. Department of Pathophysiology,Norman Bethune College of Medicine,Jilin University,Changchun 130021,China;3.Pharmacology Laboratory of Chinese Drugs,Research and Development Center,Changchun University of Chinese Medicine,Changchun 130117,China
  • Received:2012-01-21 Online:2013-05-28 Published:2013-07-01

摘要: 目的:探讨结肠安栓联合舒血宁对TNBS诱导的大鼠溃疡性结肠炎(UC)的治疗作用,阐明其活血、抗炎和调节免疫的作用机制。方法:将64只Wistar大鼠随即分为空白组(n=8),模型组(n=8),美沙拉嗪组(0.380 g?kg-1,n=8),舒血宁组(1.8 mL/kg,n=8),结肠安栓A、B组(1.215 g?kg-1,2.430 g/kg,n=8),联合给药A组(结肠安栓1.215 g/kg+舒血宁1.8 mL/kg,n=8)和联合给药B组(结肠安栓2.430 g/kg +舒血宁1.8 mL/kg,n=8)。采用TNBS诱导大鼠UC模型,造模后连续给药14 d,进行一般症状学观察,测定大鼠体质量、血液指标、淋巴细胞增殖能力,RT-PCR法检测IFN-γ和TNF-α mRAN水平,应用HE染色和免疫组织化学染色法观察大鼠结肠组织病理形态学变化和脾脏组织中CD4+表达。结果:与模型组比较,联合给药组UC大鼠的症状改善,体质量恢复;血小板(PLT)数量明显降低(P<0.05,P<0.01),血浆凝血酶原时间(PT)和延长部分凝血活酶时间(APTT)明显延长(P<0.05,P<0.01),3种炎细胞数量均有降低,IFN-γ和TNF-α mRNA表达水平明显降低,淋巴细胞刺激指数(SI)明显增高(P<0.01)。病理组织学染色,模型组大鼠结肠黏膜病理损伤最为严重,结肠黏膜可见糜烂、溃疡形成,有大量炎细胞浸润。联合给药组大鼠结肠黏膜溃疡黏膜上皮部分修复,腺体排列相对整齐,杯状细胞破损少,炎细胞浸润相对减轻。免疫组织化学染色,联合给药组大鼠脾脏组织中CD4+免疫反应阳性细胞数明显减少。结论:结肠安栓联合舒血宁对UC有治疗作用,其机制可能是活血、调节免疫从而减轻炎症反应。

关键词:  , 结肠安栓, 舒血宁, 溃疡性结肠炎, 免疫, 炎症

Abstract: Abstract:Objective To explore the therapeutic effect of suppository of Jiechang An combined with  Shuxue Ning on rats with ulcerative colitis(UC) induced with TNBS  and to clarify its mechanisms of Huoxue,anti-inflammtory,and immunomodulation.Methods 64 Wistar rats were randomly divided into control group(n=8),model group(n=8),mesalazine group(0.380 g/kg,n=8),Shuxue Ning group(1.8 mL?kg-1,n=8),suppository of Jiechang An  A and B  groups(1.215 g/kg,2.430 g/kg,n=8),suppository of Jiechang An combined Shuxue Ning A and B groups(1.215 g/kg+1.8 mL/kg and 2.430 g/kg+1.8 mL/kg,n=8).The UC model was induced by TNBS in rats.The drugs were administrated for 14 d after  modelling.The symptomatology,body weight,blood index,and proliferation ability of lymphocytes were observed.The expression levels of IFN-γ and TNF-α mRAN were detected with RT-PCR.The  pathological changes of colon tissue of rats  and the expression  of CD4+ in spleen tissue of rats were examined  by HE staining and  immunohistochemical staining. Results  Compared with model group,the symptoms  were improved  in combined drug groups,the body weights and stimulation index(SI) were increased;the platelet (PLT) amount(P<0.05 or P<0.01),the number of three kinds of inflammatory cells,the expression levels of IFN-γ,TNF-α mRNA  were decreased in combined drug groups;but the prothrombin time (PT)and partial thromboplastin time (APTT) were prolonged in combined drug groups(P<0.05 or P<0.01).Obvious   colonic mucosa damages,such as  erosion and  ulcer formation of colonic mucosa and  a large number of infiltrated inflammatory cells  in model group were found. However,the colonic mucosa damage and inflammatory infiltrating were much slighter;parts of colonic mucosa ulcer mucous membrane epithelium was repaired,and the bodies of gland arranged in good order relatively, and the  damaged cyathiform cells were decreased in combined drug groups. Immunohistochemical staining showed that the numbers of cells with positive CD4+expression  in  combined drug groups were decreased.Conclusion Suppository of Jiechang An combined with  Shuxue Ning has therapeutic effect in UC rats,and its mechanism may be related to reducing inflammatory reaction through Huoxue and immunomodulation.

Key words: suppository of Jiechang An, ShuxueNing, ulcerative colitis, immune, inflammatory

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