吉林大学学报(医学版) ›› 2013, Vol. 39 ›› Issue (3): 570-573.doi: 10.7694/jldxyxb20130330

• 基础研究 • 上一篇    下一篇

VDR基因FokⅠ多态性与不同严重程度前列腺炎发生的关联性分析

阮黎1,华兴2,李正明1   

  1. 1.广东省广州市红十字会医院,暨南大学医学院附属广州红十字会医院泌尿外科,广东 广州 510220,2.广东省广州市红十字会医院病理科,广东 广州 510220
  • 收稿日期:2012-10-19 出版日期:2013-05-28 发布日期:2013-07-01
  • 通讯作者: 阮 黎(Tel:020-34403797,E-mail:ruanli1978@126.com) E-mail:ruanli1978@126.com
  • 作者简介:阮 黎(1978-),男,湖北省荆门市人,副主任医师,医学博士,主要从事泌尿男科基础和临床研究。
  • 基金资助:

    国家自然科学基金面上项目资助课题(81272849)

Association analysis between  vitamin D receptor gene polymorphism

RUAN Li1,HUA Xing2,LI Zheng-ming1    

  1. 1.Department of Urinary Surgery,Affiliated Guangzhou Red Cross Hospital, School of Medical Science,Jinan University,Guangzhou City Red Cross Hospital of Guangdong Province,Guangzhou 510220,China;2. Department of Pathology, Guangzhou Red Cross Hospital,Guangdong Prvince,Guangzhou 510220,China
  • Received:2012-10-19 Online:2013-05-28 Published:2013-07-01

摘要: 目的:研究维生素D受体(VDR)基因启动子FokⅠ单核苷酸多态性(SNP)与不同严重程度前列腺炎(HP)的关联性,探讨遗传学因素在HP发病中的作用。方法:选择169例良性前列腺增生(BPH)并发HP患者,采用常规病理学检查将患者分为轻度组79例,中度组52例,重度组38例,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法进行 FokⅠ SNP检测,分析3组患者基因型和等位基因频数分布情况。结果:轻、中度HP患者Fok Ⅰ位点3种基因型分布符合Hardy-Weinberg平衡定律(P>0.05),重度HP患者中Fok Ⅰ位点3种基因型的分布不符合Hardy-Weinberg平衡定律(P<0.05)。FF、Ff和ff基因型在轻度和中度HP患者的分布为32%(25/79),46%(36/79);22%(18/79),23%(12/52);40%(21/52),37%(19/52);组间比较差异均无统计学意义(P>0.05)。F基因频率在轻度和中度HP患者组分别为54%(86/158)和43%(45/104),f基因频率在2组间分别为46%(72/158)和57%(59/104),组间比较差异无统计学意义(P>0.05)。结论:VDR基因FokⅠ SNP可能与不同严重程度HP发生无关联。

关键词: 组织学前列腺炎, 单核苷酸多态性, 维生素D受体

Abstract: Abstract:Objective To investigate the association between single nucleotide polymorphism (SNP) of Fok Ⅰ in vitamin D receptor (VDR) gene and different degrees of histological prostatitis(HP),and to discuss the role of genetic factors in  the onset of HP.Methods  169 patients with benign prostatic hyperplasia (BPH) complicated with  HP were selected.The mild(79 cases),moderate(52 cases) and  severe(38 cases) HP patients were distinguished by pathological  examination.Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to determine the  SNP of Fok Ⅰ of patients.The distribution of genotypic and allelic frequencies of the patients in various groups was analyzed.Results The  distribution of genotypic frequency of Fok Ⅰ site in  mild and moderate  HP patients didn’t deviate from  Hardy-Weinberg equilibrium(P>0.05).The  distribution of genotypic frequency of Fok Ⅰ gene in  severe HP patients deviated from  Hardy-Weinberg equilibrium(P<0.05).The  distribution of FF,Ff,ff genotypes  in  mild and moderate HP patients were 32%(25/79),46%(36/79);22%(18/79),23%(12/52);40%(21/52),37%(19/52), respectively;there was no significant difference between two groups(P>0.05).The  allelic frequencies of F and f were 54%(86/158),46%(72/158) in  mild HP patients  and 43%(45/104),57%(59/104) in moderate  HP patients, respectively;there was no significant difference between two groups (P>0.05).Conclusion VDR gene FokⅠ SNP may not associate with the  occurrence of different degrees of HP.

Key words: histological prostatitis, single nucleotide polymorphism, vitamin D receptor

中图分类号: 

  • R588