吉林大学学报(医学版)

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再生障碍性贫血患者外周血和骨髓中IL-17、IL-23及TGF-β
表达水平及其临床意义

李 军1,黄可欣2,陆培信3,宋雪松4,马洪喜5    

  1. 1. 吉林大学中日联谊医院血液肿瘤科,吉林  长春 130033; 2.吉林大学基础
    医学院实验平台中心, 吉林 长春 130021;3.吉林大学基础医学院生物化学与分子生物学实验中心,吉林  长春 130021;4. 吉林大
    学第一医院麻醉科,吉林  长春 130021;5. 吉林大学第一医院病理科 ,吉林  长春 130021
  • 收稿日期:2013-04-10 出版日期:2014-01-28 发布日期:2014-01-25
  • 通讯作者: 宋雪松(Tel:0431-88782563,E-mail:songxs@jlu.edu.cn); 马洪喜(Tel:0431-88782631,E-mail:497041338@qq.com) E-mail:songxs@jlu.edu.cn;497041338@qq.com
  • 作者简介:李 军(1976-),女,吉林省长春市人,主治医师,医学博士,主要从事血 液肿瘤学研究。 
  • 基金资助:

    吉林省科技厅自然科学基金资助课题(201115116)

Expression levels  of IL-17,IL-23 and TGF-β in peripheral
 blood and bone marrow of patients with aplastic anemia
and clinical significances

LI Jun1,HUANG Ke-xin2,   LU Pei-xin3,  SONG Xue-song4,  MA Hong-xi5   

  1. 1.Department of Hematology and Oncology,China-Japan Union Hospital,Jilin
      University,Changchun 130033,China; 2. Experimental Platform Center,School of Basic Med
    ical Sciences,Jilin University,Changchun 130021,China;3. Experimental Center of Biochemis
    try and Molecular Biology,School of Basic Medical Sciences,Jilin University,Changchun 130033,
    China;4. Department of Anesthesiology,First Hospital,Jilin University,Changchun 130021,China; 5. Department of Pathology,First Hospital,Jilin University,Changchun 130021,China
  • Received:2013-04-10 Online:2014-01-28 Published:2014-01-25

摘要:

目的:探讨再生障碍性贫血(AA)患者外周血和骨髓中白细胞介素17(IL-17)、
白细胞介素23(IL-23)及转化生长因子β(TGF-β)的表达水平及其临床意义,阐明AA的
发病机制。方法:随机选取50例AA患者作为实验组,30例同期体检健康人作为对照组;采
用酶联免疫吸附法(ELISA)和实时荧光定量PCR法检测外周血、骨髓中IL-17、IL-23、TGF-
β蛋白及mRNA表达水平。结果:与对照组比较,实验组患者外周血、骨髓中IL-17和IL-23
蛋白及mRNA表达水平明显升高,组间比较差异有统计学意义(P<0.01),其中以IL-1
7升高最为显著(P<0.001);实验组患者外周血和骨髓中TGF-β蛋白及mRNA表达水平明
显降低,组间比较差异亦有统计学意义(P<0.01)。实验组患者外周血和骨髓中IL-17与IL-2
3蛋白表达水平均呈正相关关系(r=0.69,P<0.05;r=0.71,P<0.05),IL-17与TGF-β蛋白表达水平呈负相关关系(r=-0.65,P<0.05),
IL-23与TGF-β蛋白表达水平呈负相关关系(r=-0.72,P<0.05)。结论:IL-17、IL-23和TGF-β可能直接参与AA的发生发展过程,三者的联
合检测可为AA患者的临床治疗提供新的思路。

关键词: 再生障碍性贫血, 骨髓, 白细胞介素17, 白细胞介素23, 转化生长因子&beta

Abstract:

Objective To explore the expression levels of IL-17,IL-23 and TGF-β
 in peripheral blood and bone marrow of the patients with aplastic anemia(AA) and its clinical significances,and to clarify the pathogenesis of AA.
Methods  50 cases of AA patients were randomly selected as experiment group and 30 cases of
healthy people at the same period were used as  control group;Enzyme-linked immunosorbent assay(ELISA) and real-time fluorescence quantitative were performed to detect the expression levels of IL-17,IL-23 and TGF-β proteins and mRNA in peripheral blood and bone marrow.
Results  Compared with control group,the protein and mRNA levels of IL-17 and IL-23 in peripheral blood and bone marrow of the patients in experiment group were significantly increased,and the differences between two groups were statistically significant(P<0.01),among them,the  elevated IL-17 was significant(P<0.001);the protein and mRNA levels of TGF-β in peripheral blood and bone marrow of the patients in experiment group were significantly
 decreased,and the differences between two groups  were also statistically significant (P<0.01).The protein and mRNA levels of  IL-17 and IL-23
 in peripheral blood and bone marrow of the patients in experiment group had  positive correlation (r=0.69,P=0.041;r=0.71,P<0.05),while the pr
otein  levels of  IL-17  and TGF-β had  negative correlation(r=-0.65,P<0.05);and the potein levels of IL-23 and TGF-β had  negative correlati
on(r=-0.72,P<0.05).Conclusion IL-17,IL-23 and TGF-β may participate in the occurrence and development of AA,and the combined dete
ction of the three indexes provides the new ideas for clinical treatment of AA.

Key words: aplastic anemia, bone marrow, interleukin-17, interleukin -23, transforming growth factor-&beta

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