关键词: 再生障碍性贫血, 骨髓, 白细胞介素17, 白细胞介素23, 转化生长因子&beta

Abstract:

Objective To explore the expression levels of IL-17,IL-23 and TGF-β
 in peripheral blood and bone marrow of the patients with aplastic anemia(AA) and its clinical significances,and to clarify the pathogenesis of AA.
Methods  50 cases of AA patients were randomly selected as experiment group and 30 cases of
healthy people at the same period were used as  control group;Enzyme-linked immunosorbent assay(ELISA) and real-time fluorescence quantitative were performed to detect the expression levels of IL-17,IL-23 and TGF-β proteins and mRNA in peripheral blood and bone marrow.
Results  Compared with control group,the protein and mRNA levels of IL-17 and IL-23 in peripheral blood and bone marrow of the patients in experiment group were significantly increased,and the differences between two groups were statistically significant(P<0.01),among them,the  elevated IL-17 was significant(P<0.001);the protein and mRNA levels of TGF-β in peripheral blood and bone marrow of the patients in experiment group were significantly
 decreased,and the differences between two groups  were also statistically significant (P<0.01).The protein and mRNA levels of  IL-17 and IL-23
 in peripheral blood and bone marrow of the patients in experiment group had  positive correlation (r=0.69,P=0.041；r=0.71，P<0.05),while the pr
otein  levels of  IL-17  and TGF-β had  negative correlation(r=－0.65,P<0.05);and the potein levels of IL-23 and TGF-β had  negative correlati
on(r=－0.72,P<0.05).Conclusion IL-17,IL-23 and TGF-β may participate in the occurrence and development of AA,and the combined dete
ction of the three indexes provides the new ideas for clinical treatment of AA.


Key words: aplastic anemia, bone marrow, interleukin-17, interleukin -23, transforming growth factor-&beta