关键词: 系统性, 小鼠, 近交系, 粒-巨噬细胞集落形成单位, 单核细胞

Abstract: Objective To study the proliferation of colony-forming units of granulocytes and macrophages (CFU-GM) and relationship with pathogenesis in BXSB lupus mice. Methods The formation of CFU-GM of bone marrow mononuclear cells (BMMC) of 4, 8, 12, 16 and 20-week-old male BXSB mice cultured in vitro with methylcellulose semisolid culture system was observed, the peripheral blood monocytes (PBMC) were counted and the urinary protein was test at the same time. C57BL mice with same ages were used as normal controls. Results The urinary protein emerged since 12 weeks in male BXSB, but remained negative in 4, 8-week-old male BXSB and C57BL with any ages. The CFU-GM was （96.00±18.11）/2×10⁵ BMMC, and peripheral blood monocytes reached （111.33±10.97）×10⁶•L^-1 in 8-week-old male BXSB mice, the counts were significantly higher compared with those of 4-week-old male BXSB mice and normal C57BL mice (P<0.05), and increased progressively in older BXSB mice. Conclusion The autoimmune disease of male BXSB mice may be caused by monocytosis which comes from the abnormal CFU-GM in the bone marrow.

Key words: systemic, mice, inbred strains, granulocytes and macrophages colony-forming units, monocytes 