玉米活性多糖对糖尿病小鼠的降血糖作用

doi:国家“十五”重大科技攻关专项（2001BA501A

玉米活性多糖对糖尿病小鼠的降血糖作用

张艳荣，陈丽娜，王大为^*

吉林农业大学食品工程学院，吉林长春130118

收稿日期:2004-04-30 修回日期:1900-01-01 出版日期:2005-11-28 发布日期:2005-11-28
通讯作者: 王大为

Action of lowering blood sugar levels of corn active polysaccharide on diabetic mice

ZHANG Yan-rong, CHEN Li-na, WANG Da-wei^*

College of Food Engineering，Jilin Agricultural University，Changchun 130118,China

Received:2004-04-30 Revised:1900-01-01 Online:2005-11-28 Published:2005-11-28
Contact: WANG Da-wei

摘要/Abstract

摘要： 目的：研究玉米活性多糖（CAP）对糖尿病小鼠的降血糖作用。方法：小鼠50只随机分为空白对照组、四氧嘧啶模型组和CAP 3.50、1.75、0.88 g·kg ^-1 3个剂量组，每天给予CAP 1次，连续15 d，于第6天除空白对照组外，各组小鼠均尾静脉注射四氧嘧啶生理盐水溶液 80 mg·kg^-1，空白对照组尾静脉注射同体积生理盐水。末次给药前16 h将所有各组小鼠饥饿，末次给药后1 h，于小鼠眼球采血，分离血清，采用全自动生化分析仪测血糖含量；立即处死小鼠，取肝脏测小鼠肝糖元含量。另取60只小鼠，分组方法同上，每天给予CAP 1次，连续10 d。除空白对照组外,其余各组均在末次给予CAP后1 ｈ腹腔注射盐酸肾上腺素250 μg·kg^-1，30 min后断头取血,测血糖含量，取肝组织测肝糖元含量。结果：CAP 3.50 g·kg^-1对四氧嘧啶糖尿病小鼠血糖升高有明显的抑制作用（P<0.05），对四氧嘧啶糖尿病小鼠肝糖元含量有明显的降低作用（P<0.05），且CAP 3.50 g·kg^-1 优于1.75 g·kg^-1。3.50、 1.75和 0.88 g·kg^-1剂量组对肾上腺素小鼠血糖升高均有明显的抑制作用（P<0.001、P<0.01和P <0.05），对肾上腺素造成小鼠肝糖元降低有明显的升高作用，且3.50 g·kg^-1优于 1.75 g·kg^-1。结论：CAP对四氧嘧啶致糖尿病小鼠及肾上腺素血糖升高小鼠具有明显的降血糖作用。

关键词: 糖尿病, 实验性, 降血糖药

Abstract: Objective To study the action of corn active polysaccharide (CAP) on blood sugar levels in diabetic mice.Methods Fifty mice were randomly divided into control group, alloxan model group, CAP 3.50,1.75, 0.88 g·kg^-1 groups (n=10). Mice in each group were exhibited with CAP once a day for 15 d，on the sixth day,mice in every group were injected with alloxan physiological salt solution 80 mg·kg^-1 in caudal vena except control group, mice in control group were injected with physiological salt solution at the same volume. 16 h before the last exhibition, mice in every group were starved. 1 h before the last exhibition, blood was extracted in eyeballs, sera were separated and the contents of blood sugar were determined by auto-biochemistry analyzer, mice were killed at once. The liver samples were obtained and the contents of liver glycogen were determined. Another 60 mice were divided into 5 groups with the method men tioned above. Each group was exhibited once a day for 10 d continuously. 1 h after the last exhibition, except control group, the mice were injected with adrenalin hydrochloride 250 μg·kg^-1 through abdominal cavity, after30 min, blood was extracted by decapitation, and the contents of blood sugar and liver glycogen were determined by auto-biochemistry analyzer.Results CAP 3.50　g·kg^-1 had obvious inhibitory effect on the raise of blood sugar in diabetic mice （model group）（P＜0.05）, it had obvious action on reducing the contents of liver glycogen in diabetes mice（model group）（P＜0.05）,and 3.50　g·kg^-1 was superior to 1.75 g·kg^-1. CAP with different doses had obvious actions on inhibiting the raise of blood sugar in adrenine mice（model group）（P＜0.001, P＜0.01, P＜0.05）,and had obvious action on increasing the reduction of liver glycogen on adrenine mice（model group）,and 3.50 g·kg^-1 was superior to 1.75 g·kg^-1. Conclusion CAP has the function of lowering blood sugar levels in alloxan diabetic mice and hyperglycemia mice induced by adrenine.

Key words: diabetes mellitus, experimental, hypoglycemic agents

中图分类号:

Q536

张艳荣，陈丽娜，王大为. 玉米活性多糖对糖尿病小鼠的降血糖作用[J]. J4, 2005, 31(6): 846-848.

ZHANG Yan-rong, CHEN Li-na, WANG Da-wei. Action of lowering blood sugar levels of corn active polysaccharide on diabetic mice[J]. J4, 2005, 31(6): 846-848.

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