关键词: 辐射防护, 造血系统, 细胞周期

Abstract: Abstract:Objective To explore the protective effect of the polysaccharides of tricholoma matsutake(PTM) on hematopoietic function of X-rays irradiated mice.Methods Fifty ICR mice were divided into five groups randomly，10 each group．The mice in normal control group(NC) and radiation control group(IC) were gaster-poured by 0.9% sodium chloride solution．The mice in PTM groups were gaster-poured by three different doses PTM (400,800,1200 mg•kg^-1) for 7 d.The mice all groups except NC group were irradiated once with 2.0 Gy X-rays on the eighth day.The conony forming units-spleen (CFU-S),fibrolast colony forming unit (CFU-F),cell cycle of bone marrow cells and micronucleus rate of bone marrow polychromalic erythrocytes (PCE) were detected after radiation for 24 h.Results Compared with IC group,the number of CFU-S in PTM groups decreased significantly(P<0.05 or P<0.01)and the number of CFU-F increased markedly(P<0.05 or P<0.01);in high and moderate doses PTM the micronucleus rates of bone marrow PCE and the percentage of bone marrow cells at G₁ phase decreased signficantly( P<0.05 or P<0.01); the percentage of cells at S phase increased signficantly( P<0.05 or P<0.01).　Conclusion PTM has an obvious protective effect on hematopoietic function of X-rays irradiated mice.

Key words: radioprotection, hematopoietic system, cell cycle