关键词: 雌激素受体β, 乳腺

Abstract: Objective To explore the cellular location of ER subtypes (ERα and ERβ) in normal human mammary gland and provid the foundation for exploring the relationship between ERα,ERβ and development of normal mammary gland. Methods Using immunohistochemistry,the expressions and cellular distribution of ERα and ERβ in the normal tissues of 16 patients who had accepted reduction mammoplasty specimens in the Affiliated Hospital of University Laval were detected.Results ERα immunoreactivity was detected in the nuclei of epithelial cells lining lobules and ducts.The positive cells located in the inner layer of the two epithelial layers in the lobules and intralobular ducts,but in the interlobular ducts the positive cells located in the outer layer.Although ERβ was also seen in these cells,there was irregularity of its distribution.Weak to moderate cytoplastic staining of ERβ in epithelial cells of lobules and ducts were fonud.Occasional nuclear staining was seen in the stromal cells,endothelial cells and lymphocytes.The percentage of ERβ positive expression in the epithelial cells was much higher than that of ERα,there was markedly difference between them (t=29.789，P<0.01 ).The positive expression rate of ERβ in the mammary gland was much higher than that of ERα,there was markedly difference between them (χ²=8.127,P＜0.05). Conclusion ER subtypes have distinct distribution patterns in the normal mammary gland.The widespread distribution of ERβ suggests that it may be the dominant ER in the mammary gland.

Key words: estrogen receptor beta, mammary glands