关键词: 胰岛, β细胞, 细胞培养

Abstract: Objective To study the effect of CB₄V infection on human embryonic islet cell. Methods Human embryonic islet cell was infected by CB₄V in vitro, the morphological change of that was observed by electro microscope, and insulin releasing test by sugar stimulation was also proceeded. Results CB₄V could attack islet directly ,break the envelope of islet, invade β cell and proliferated in β cell. The quantity of insulin releasing stimulated by sugar increased 24 h after infection, then decreased gradually. The quantities of insulin releasing stimulated by hypoglycemia and hyperglycemia in infected group were (64.50±9.20)mU•L^-1 and (87.85±8.50) mU•L^-1,respectively,24 h later after infection, which showed significant difference compared with the control group (P<0.05). From 48 h to 6 d, the quantities of insulin releasing stimulated by hypoglycemia and hyperglycemia were much lower than that of control group (P<0.05，P<0.01).Conclusion CB₄V can infect human embryonic islet cell in vitro, and damage the function of islet β cell of secreting insulin.

Key words: islets of langerhans, β cell, cell culture