关键词: 纤维蛋白, 胶原, Ⅱ型

Abstract: Objective To detect collagen Ⅱ in constructed articular cartilage by three-dimensional culturing system using collagen and human fibrin as supporter. Methods Chondrocytes were isolated from articular cartilage of 2-week-old rabbit, digested by collagenase Ⅱ, and mixed with bovine collagen I, human blood freeze-dry fibrinogen and thrombosin according to a certain proportion,and were cultured in vitro. Biopsies was analyzed by Masson stain, hybridization in situ using oligonucleotied probe of collagen Ⅱ and immunohistochemistry test of collagen Ⅱ after 3 weeks. Results After 3 weeks, all cells were viable, formed cartilage lacunae, appeared homologous cell cluster and secreted extracellular matrix that could be stained with Masson stain. In situ hybridization assay showed that chondrocytes expressed of collagen Ⅱ mRNA. It was demonstrated that chondrocytes could secrete collagen Ⅱ with immunohistochemistry method. Conclusion Chondrocytes still secrete collagen Ⅱin three-dimensional culturing system using collagen and human fibrin as supporter. Collagen Ⅱ is one of the most important component of cartilage tissue.

Key words: fibrin, collagen type Ⅱ