盐酸异丙肾上腺素诱发大鼠心肌缺血性纤维化后肝脏损伤的拉曼光谱分析

doi:10.13481/j.1671-587x.20150329

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (03): 584-587.doi: 10.13481/j.1671-587x.20150329

盐酸异丙肾上腺素诱发大鼠心肌缺血性纤维化后肝脏损伤的拉曼光谱分析

黄宇欣^1,2, 高海成³, 刘凯³, 苗春生³

1. 吉林大学理论化学研究所理论化学计算国家重点实验室, 吉林长春 130012;
2. 长春工业大学基础科学学院物理教研室, 吉林长春 130012;
3. 吉林大学药学院临床药学教研室, 吉林长春 130021

收稿日期:2014-10-29 发布日期:2015-08-01
通讯作者: 苗春生,副高级实验技师(Tel:0431-85619702,E-mail:miaocs@jlu.edu.cn) E-mail:miaocs@jlu.edu.cn
作者简介:黄宇欣(1981-),女,吉林省长春市人,讲师,在读吉林大学理论化学研究所博士后,主要从事光谱谱图分析方面的研究。
基金资助:
吉林省科技厅医药大品种专项资金资助课题(YYZX201259);吉林省科技厅科学计划项目资助课题(201101092, 20140312002ZG);吉林大学基本科研费资助课题(409020720084)

Analysis of Raman spectroscopy for Iso-induced liver injury after myocardial fibrosis of ischemiain rats

HUANG Yuxin^1,2, GAO Haicheng³, LIU Kai³, MIAO Chunsheng³

1. Institute of Theoretical Chemistry, National Key Laboratony of Theoretical Chemistry Calculation, Jilin University, Changchun, 130012, China;
2. Department of Physics, School of Fundamental Sciences, Changchun University of Technology, Changchun, 130012, China;
3. Department of Clinical Pharmacy, School of Pharmacy, Jilin University, Changchun 130021, China

Received:2014-10-29 Published:2015-08-01

摘要/Abstract

摘要：

目的:探讨盐酸异丙肾上腺素(Iso)诱发大鼠心肌缺血性纤维化后拉曼光谱对肝脏损伤的分析,为拉曼光谱的应用提供依据。方法:20只Wistar 大鼠随机分为对照组、Iso-4h模型组、Iso-24h模型组和Iso-1周模型组(n=5);除对照组外,其余各组大鼠均一次性皮下多点注射Iso。HE染色分析心脏和肝脏的病理学改变;紫外光谱和拉曼光谱分析肝脏组织的蛋白吸收谱。结果:病理学观察,对照组大鼠心肌细胞排列整齐,横纹清晰,胞核明显,无细胞肿胀;Iso-24h组大鼠心肌出现较多的坏死,局限于心内膜下;Iso-1周组大鼠心肌出现界限清晰、多发散在坏死灶,坏死灶内有旺盛的成纤维细胞增生,并可见一定量的胶原纤维。对照组大鼠肝小叶界限清晰,无坏死灶;Iso各组大鼠肝小叶内有灶性坏死区,肝细胞出现水样变性,且随着Iso注射时间的延长,肝脏病变程度加重。紫外检测,与对照组比较,Iso-24h组大鼠心脏组织吸收峰向左或向右移动1个单位,Iso-1周组由1个峰位趋于正常;与对照组比较,Iso-24h组大鼠肝脏组织吸收峰移动了2个单位,Iso-1周组吸收峰位逐渐趋于平稳。拉曼光谱法,与对照组比较,Iso-24h组大鼠肝组织仅在1 590 nm处见明显吸收峰,Iso-1周组未检测到相同吸收峰。结论:Iso诱发心肌损伤时,肝脏亦有损伤;拉曼光谱是更为灵敏的检测肝损伤的方法。

关键词: 盐酸异丙肾上腺素, 心肌缺血, 拉曼光谱法, 肝脏

Abstract:

Objective To investigate the effect of Raman spectroscopy for isoprenaline hydrochloride(Iso)-induced liver injury after myocardial fibrosis of ischemia in the rats, and to provide the basis for the application of Raman spectroscopy. Methods 20 Wistar rats were randomly divided into control group, Iso-4h group, Iso-24h group, and Iso-1 week group (n= 5).Except the normal group, the rats in other groups were subcutaneously injected with Iso.The pathological changes of heart and liver tissues were analyzed by HE staining.The absorption spectra of proteins in the liver tissue was analyzed by UV and Raman spectroscopy. Results The pathological observation Results showed that the cardiomyocytes of the rats in control group arranged in neat rows with clear stripes, obvious nucleus and no cell swelling;the cardiomyocytes of the rats in Iso-24h group appeared the more necrosis limting under endocardium;the myocardium of the rats in Iso-1 week group appeared the multiple scattered necrosis with fibroblasts and collagen fiber.The hepatic lobules of the rats in control group had clear boundaries without necrosis;the hepatic lobules of the rats in Iso groups had focal necrosis, and the liver cells appeared watery degeneration;with the prolongation of the Iso injection time, the degree of liver lesion was serious.The UV spectroscopy showed that the myocardial protein spectrum of the rats in Iso-24h group moved 1 unit to the left or the right, and the spectrum in Iso-1 week group became nromal compared with control group;the liver protein spectrum of the rats in Iso-24h group moved 2 units, and the spectrum in Iso-1 week gradually stabilized compared with control group.The Raman spectrocopy showed that the absorption peak of liver tissue in Iso-24h group appeared at 1 590 nm, but it wasn't found in Iso-1 week group. Conclusion Liver damage is found when Iso induces myocardium injury, and Raman spectroscopy is a more sensitive detection method for liver damage.

Key words: isoprenaline hydrochloride, myocardial ischemia, Raman spectroscopy, liver

中图分类号:

R363

黄宇欣, 高海成, 刘凯, 苗春生. 盐酸异丙肾上腺素诱发大鼠心肌缺血性纤维化后肝脏损伤的拉曼光谱分析[J]. 吉林大学学报(医学版), 2015, 41(03): 584-587.

HUANG Yuxin, GAO Haicheng, LIU Kai, MIAO Chunsheng. Analysis of Raman spectroscopy for Iso-induced liver injury after myocardial fibrosis of ischemiain rats[J]. Journal of Jilin University Medicine Edition, 2015, 41(03): 584-587.

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盐酸异丙肾上腺素诱发大鼠心肌缺血性纤维化后肝脏损伤的拉曼光谱分析

Analysis of Raman spectroscopy for Iso-induced liver injury after myocardial fibrosis of ischemiain rats

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