吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (01): 48-53.doi: 10.13481/j.1671-587x.20150110

• 基础研究 • 上一篇    下一篇

瘦素对乳腺癌MCF-7细胞增殖和凋亡的影响及其作用机制

李矿发, 庞雪利, 黄云秀, 魏兰, 苏敏, 陈婷梅   

  1. 重庆医科大学 教育部临床检验诊断学重点实验室, 重庆 400016
  • 收稿日期:2013-10-25 发布日期:2015-01-30
  • 通讯作者: 陈婷梅,教授,硕士研究生导师(Tel:023-68485938,E-mail:chentingmei@sohu.com) E-mail:chentingmei@sohu.com
  • 作者简介:李矿发(1988-),男,山西省运城市人,医学硕士,主要从事肿瘤微环境与肿瘤转移、新生血管生成和肿瘤免疫抑制等方面的研究。
  • 基金资助:

    国家自然科学基金面上项目资助课题(81272544)

Effects of leptin on proliferation and apoptosis of breast carcinoma MCF-7 cells and its mechanism

LI Kuangfa, PANG Xueli, HUANG Yunxiu, WEI Lan, SU Min, CHEN Tingmei   

  1. Key Laboratory of Laboratory Medical Diagnostics, Ministry of Eduction, Chongqing Medical University, Chongqing 400016, China
  • Received:2013-10-25 Published:2015-01-30

摘要:

目的: 研究瘦素对乳腺癌MCF-7细胞增殖和凋亡的影响,并探讨其作用机制。方法: 选取处于对数生长期的MCF-7细胞,随机分为对照组和20、40、80 μg·L-1瘦素处理组。CCK8试剂盒检测各组MCF-7细胞增殖率;流式细胞术检测各组MCF-7细胞凋亡率;RT-PCR和Western blotting法分别检测各组MCF-7细胞bcl-2和bax的mRNA和蛋白表达水平;在抗凋亡作用的信号通路筛选实验中采用Western blotting法检测不同信号通路抑制剂处理组MCF-7细胞p-AKT和bcl-2的蛋白表达水平。结果: 与对照组比较,不同剂量瘦素处理组MCF-7细胞增殖率升高(P<0.05),且呈剂量依赖性,不同剂量瘦素组之间比较差异也有统计学意义(P<0.05);随着瘦素作用剂量增加,不同剂量瘦素处理组细胞凋亡率逐渐降低,与对照组比较差异有统计学意义(P<0.05);与对照组比较,不同剂量瘦素处理组MCF-7细胞 bcl-2 mRNA和蛋白表达水平增加(P<0.05),bax mRNA和蛋白表达水平降低(P<0.05);与瘦素组比较,PI3K-AKT信号通路抑制剂LY294002处理组MCF-7细胞的p-AKT和bcl-2蛋白表达水平明显下降(P<0.05)。结论: 瘦素对乳腺癌MCF-7细胞有促进增殖和拮抗凋亡的作用,其抗凋亡效应与通过细胞信号通路PI3K-AKT促进bcl-2表达有关。

关键词: 瘦素, 乳腺肿瘤, 细胞凋亡, 信号通路

Abstract:

Objective To observe the effects of leptin on the proliferation and apoptosis of the breast carcinoma MCF-7 cells, and to explore the mechanism. Methods The MCF-7 cells at logarithm growth phase were selected and randomly divided into control group and different doses (20, 40, 80 μg·L-1) of leptin groups.The proliferation rates of the MCF-7 cells in various groups were determined by CCK8 assay kit;the apoptotic rates of the MCF-7 cells in various groups were analyzed by flow cytometry;the mRNA and protein expressions of bcl-2 and bax of the MCF-7 cells in various groups were detected by RT-PCR and Western blotting methods;the protein expressions of p-AKT and bcl-2 in the MCF-7 cells in different cell signaling pathway inhibitor treatment groups were tested by Western blotting method. Results Compared with control group, the proliferation rates of the MCF-7 cells in leptin groups were increased in a dose-dependent manner(P<0.05);the apoptotic rates of the MCF-7 cells in leptin groups were decreased(P<0.05);the mRNA and protein expression levels of bax in the MCF-7 cells in leptin groups were decreased(P<0.05), and the mRNA and protein expression levels of bcl-2 were increased(P<0.05);compared with leptin group, the protein expressions of p-AKT and bcl-2 in PI3K-AKT inhibitor LY294002 group were markedly decreased(P<0.05). Conclusion Leptin can increase the proliferation and inhibit the apoptosis of breast carcinoma MCF-7 cells, and the mechanism of anti-apoptosis may be releted to the up-regulation of bcl-2 via PI3K-AKT signaling pathway.

Key words: leptin, breast carcinoma, apopotosis, signaling pathway

中图分类号: 

  • R392-33