不同人群乳头瘤病毒DNA分型的检测及其临床意义

doi:10.13481/j.1671-587x.20150622

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (06): 1211-1214.doi: 10.13481/j.1671-587x.20150622

不同人群乳头瘤病毒DNA分型的检测及其临床意义

刘洋¹, 陈柯霖², 李翔燕¹, 李清¹, 李晶华³

1. 北京天坛医院皮肤科, 北京 100050;
2. 北京天坛医院检验科, 北京 100050;
3. 北京天坛医院妇产科, 北京 100050

收稿日期:2015-06-15 发布日期:2016-01-11
通讯作者: 李晶华,副主任医师(Tel:010-67096610,E-mail:jhlee0927@163.com) E-mail:jhlee0927@163.com
作者简介:刘洋(1972-),女,吉林省四平市人,医师,医学硕士,主要从事皮肤病和性病方面的研究。
基金资助:
国家自然科学基金资助课题(81101969)

Detection of human papillomavirus DNA genotypes in different populations and its clinical significance

LIU Yang¹, CHEN Kelin², LI Xiangyan¹, LI Qing¹, LI Jinghua³

1. Department of Dermatology, Beijing Tiantan Hospital, Beijing 100050, China;
2. Department of Laboratory Medicine, Beijing Tiantan Hospital, Beijing 100050, China;
3. Department of Gynaecology and Obstetrics, Beijing Tiantan Hospital, Beijing 100050, China

Received:2015-06-15 Published:2016-01-11

摘要/Abstract

摘要：

目的: 分析尖锐湿疣(CA)患者及无症状的高危自检者、正常体检者人乳头瘤病毒(HPV)的DNA分型检测结果及其临床意义。方法: 选择门诊就诊的CA患者527例(CA组)、高危性行为自检者208例(HR组)和正常体检者197名(PE组),应用凯普导流杂交技术对3组人群样本进行HPV DNA分型检测,分析各组HPV检出阳性率、HPV优势型别分布和HPV亚型多重感染情况。结果: CA组患者HPV检出阳性率为98.29%,HPV感染的优势型别为6、11、33和52型;HR组患者HPV检出阳性率为26.44%,HPV感染的优势型别为6、11、33和66型;PE组人群HPV检出阳性率为5.58%,HPV感染的优势型别为6和11型。HR和PE组HPV检出阳性率比较差异有统计学意义(P<0.05);CA组患者HPV多重型别混合感染检出阳性率为36.43%,HR组多重型别混合感染检出阳性率为5.77%,二者比较差异有统计学意义(P<0.01);CA组患者多重型别混合感染以二重感染阳性率最高,为18.41%;三重混合感染率次之,为11.20%;最多重感染为七重型别混合感染,阳性率为0.38%。结论: 3组人群的HPV感染均以低危型6和11型最多见;高危自检者与正常体检者两者在临床上均无症状,但前者HPV检出阳性率明显高于后者;CA组患者HPV混合型别感染阳性率明显高于HR组,且多以二重或三重型别混合感染形式多见。

关键词: 尖锐湿疣, 人乳头瘤病毒, 基因分型

Abstract:

Objective To analyze the detection results and clinical significance of human papillomavirus(HPV) DNA genotypes in the patients with condyloma acuminate(CA) and high risk(HR) population of CA and normal population of physical examination(PE). Methods 527 cases of CA patients (CA group), 208 cases of HR (HR group) and 197 cases of normal PE (PE group) in the outpatient were selected. The human HPV DNA genotypes in 932 samples of different population were examined with HybriMax; the positive detection rates, the preponderance types of HPV and the multiple genotype infection in three groups were analyzed. Results The HPV positive rate in CA group was 98.29% and the preponderance types were HPV 6,11,33,and 52 genotypes. The HPV positive rate in HR group was 26.44% and the preponderance types were HPV 6,11,33,and 66 genotypes. The HPV positive rate in PE group was 5.58% and the preponderance types were HPV 6 and 11 genotypes. The difference in the positive detection rates of HPV between HR and PE groups had statistical significance(P<0.05).The positive rates of multiple genotype infection in CA group and HR group were 36.43% and 5.77%, respectively, and there was significant difference (P<0.01);the positive rate of double infection was the highest (18.41%), followed by the positive rate of triple heavy infection (11.20%) and the positive rate of septuple heavy infection (0.38%). Conclusion The HPV 6 and HPV 11 of low-risk HPV genotypes are the most common genotypes in different population. The HR group and PE group are all asymptomatic but the positive detection rate of HPV in HR group is higher than that in PE group. The positive rate of HPV multiple genotypes infection in CA group is higher than that in HR group, and the double infection or triple heavy infection are the most common infection.

Key words: condyloma acuminata, human papillomavirus, genotype

中图分类号:

R446.52

刘洋, 陈柯霖, 李翔燕, 李清, 李晶华. 不同人群乳头瘤病毒DNA分型的检测及其临床意义[J]. 吉林大学学报(医学版), 2015, 41(06): 1211-1214.

LIU Yang, CHEN Kelin, LI Xiangyan, LI Qing, LI Jinghua. Detection of human papillomavirus DNA genotypes in different populations and its clinical significance[J]. Journal of Jilin University Medicine Edition, 2015, 41(06): 1211-1214.

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不同人群乳头瘤病毒DNA分型的检测及其临床意义

Detection of human papillomavirus DNA genotypes in different populations and its clinical significance

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