吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (05): 892-896.doi: 10.13481/j.1671-587x.20160510

• 基础研究 • 上一篇    下一篇

载顺铂联合氯喹聚乳酸静电纺丝膜对口腔鳞状细胞癌CAL-27细胞增殖的抑制作用

周丽佳1,2, 徐召南1, 毕也1, 杨鹤1, 张泽兵1, 王舒煜1, 贾杰3   

  1. 1. 吉林大学口腔医院病理科, 吉林 长春 130021;
    2. 大庆医学高等专科学校临床医学系, 黑龙江 大庆 163312;
    3. 吉林大学中日联谊医院电诊科, 吉林 长春 130033
  • 收稿日期:2016-01-24 出版日期:2016-09-28 发布日期:2016-09-29
  • 通讯作者: 贾杰,副主任医师(Tel:0431-84997674,E-mail:1165158994@qq.com) E-mail:1165158994@qq.com
  • 作者简介:周丽佳(1989-),女,黑龙江省绥化市人,医学硕士,主要从事口腔肿瘤学方面的研究。
  • 基金资助:

    吉林省科技厅自然科学基金资助课题(20150101174JC);吉林省卫计委科研基金资助课题(20132004)

Inhibitory effect of poly(lactic acid) electrospun membranes loaded with cisplatin and chloroquine on proliferation of oral squamous cell carcinoma CAL-27 cells

ZHOU Lijia1,2, XU Zhaonan1, BI Ye1, YANG He1, ZHANG Zebing1, WANG Shuyu1, JIA Jie3   

  1. 1. Department of Pathology, Hospital of Stomatology, Jilin university, Changchun 130021, China;
    2. Department of Clinical Medicine, Daqing Medcial College, Daqing 163312, China;
    3. Department of Eelectronic Diagnosis, China-Japan Union Hospital, Jilin University, Changchun 130033, China
  • Received:2016-01-24 Online:2016-09-28 Published:2016-09-29

摘要:

目的:观察载药聚乳酸(PLA)静电纺丝膜对口腔鳞状细胞癌CAL-27细胞的作用,探讨预防口腔癌术后复发的方法。方法:利用静电纺丝技术制备载顺铂(DDP)PLA静电纺丝膜(DDP/PLA膜)、载氯喹(CQ)/顺铂/氯喹PLA静电纺丝膜(CQ/DDP/CQ/PLA膜,复合膜)和载氯喹/顺铂PLA静电纺丝膜(CQ/DDP/PLA膜,共混膜)。通过扫描电镜(SEM)观察3种载药PLA静电纺丝膜的表面形态;紫外分光光度分析法测定PLA静电纺丝膜的降解速率;激光共聚焦显微镜观察自噬特异性蛋白LC3-Ⅱ在CAL-27细胞中的表达情况;MTT法检测CAL-27细胞生存率。结果:SEM观察,DDP/PLA膜、复合膜和共混膜纳米纤维连续,表面光滑,直径较均匀;载药PLA静电纺丝膜在21 d左右降解;第1天DDP/PLA膜、复合膜和共混膜对CAL-27细胞杀伤作用不明显,第3天以后,DDP/PLA膜组、复合膜组及共混膜组CAL-27细胞的生存率明显降低(P<0.05);复合膜组和共混膜组LC3-Ⅱ荧光亮度均低于DDP/PLA膜组。结论:具有缓释效果的CQ/DDP/PLA纳米静电纺丝膜提高了CAL-27细胞对DDP的敏感性,增强了DDP对口腔癌CAL-27细胞的杀伤作用。

关键词: 静电纺丝, 自噬, 顺铂, 氯喹, 口腔鳞状细胞癌

Abstract:

Objective: To investigate the effect of poly(lactic acid) (PLA) electrospun membranes loaded with cisplatin and chloroquine on the oral squamous cell carcinoma CAL-27 cells,and to explore the method to prevent the recurrence of oral cancer. Methods: The DDP/PLA membranes,CQ/DDP/CQ/PLA membranes and CQ/DDP/PLA membranes were prepared by electrospinning. Then the micro morphology of three kinds of membranes were observed by scanning electron microscope(SEM);the degradation rate of PLA membrane was measuredby UV spectrophotometric. The LC3-Ⅱ expression level in CAL-27 cells was detected by laser scanning confocal microscope.The survival rate of CAL-27 cells was detected by MTT method. Results: The SEM results showed that the nanofibers of DDP/PLA,CQ/DDP/PLA and CQ/DDP/CQ/PLA membranes were continuous and smooth with uniform diameters. The degrated time of membranes was about 21 d. The MTT result showed that compared with control group,at first,the effects of cell killing of DDP/PLA membranes, CQ/DDP/CQ/PLA membranes and CQ/DDP/PLA membranes were not obvious; as the extension of time, the survival rates of CAL-27 cells in DDP/PLA membranes group,CQ/DDP/CQ/PLA membranes group and CQ/DDP/PLA membranes group were decreased(P<0.05). The immunofluorescence results showed that the fluorescence intensity of LC3-Ⅱ in CQ/DDP/CQ/PLA membranes group and CQ/DDP/PLA membranes group were lower than that in DDP/PLA membranes group. Conclusion: CQ/DDP/PLA membranes with sustained-release effect can increase the sensitivity of CAL-27 cells to DDP and enchance the killer effect of DDP on the CAL-27 cells.

Key words: electrospinning, autophagy, cisplatin, chloroquine, oral squamous cell carcinoma

中图分类号: 

  • R780.2