吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (03): 551-555.doi: 10.13481/j.1671-587x.20160325

• 临床研究 • 上一篇    下一篇

慢性乙肝病毒感染各阶段患者外周血单个核细胞中IL-22mRNA的表达水平及其临床意义

陈洪涛, 杨智, 吴诗品   

  1. 暨南大学第二临床医学院 深圳市人民医院感染内科, 广东 深圳 518020
  • 收稿日期:2015-09-25 发布日期:2016-06-17
  • 通讯作者: 吴诗品,主任医师,硕士研究生导师(Tel:0755-22942578,E-mail:wupoem@126.com) E-mail:wupoem@126.com
  • 作者简介:陈洪涛(1977-),男,湖北省丹江口市人,主治医师,医学硕士,主要从事感染病学基础和临床方面的研究。
  • 基金资助:

    广东省卫生厅医学科研基金资助课题(D2011283)

Expression levels of IL-22 mRNA in PBMCs of patients at different HBV infection phases and their significances

CHEN Hongtao, YANG Zhi, WU Shipin   

  1. Department of Infectious Diseases, Shenzhen People's Hospital, Second Clinical Medical College, Jinan University, Shenzhen 518020, China
  • Received:2015-09-25 Published:2016-06-17

摘要:

目的: 分析慢性乙型病毒性肝炎(CHB)、慢加急性/亚急性重型肝炎(重型肝炎)、乙肝肝硬化和乙型肝炎病毒(HBV)相关性原发性肝癌(HCC)患者外周血单个核细胞(PBMCs)中白细胞介素22(IL-22)mRNA的表达水平及其可能的临床意义。方法: 收集20名健康对照者(健康对照组)、33例CHB中度患者(CHB中度组)、21例CHB重度患者(CHB重度组)、16例重型肝炎患者(重型肝炎组)、16例乙肝肝硬化患者(肝硬化组)和40例HCC患者(HCC组)的临床资料。RT-PCR半定量分析各组研究对象PBMCs中IL-22mRNA表达水平;Pearson相关分析法分析IL-22mRNA表达水平与临床重要检测指标的相关性。结果: CHB中度、CHB重度和肝硬化组患者PBMCs中IL-22mRNA表达水平均高于健康对照组(P<0.001);重型肝炎组患者PBMCs中IL-22mRNA表达水平与健康对照组比较差异无统计学意义(P=0.830);HCC组患者PBMCs中IL-22mRNA表达水平明显低于健康对照组(P<0.001)。CHB重度组患者和重型肝炎组患者PBMCs中IL-22mRNA表达水平与总胆红素(TB)水平呈明显负相关关系(r=-0.383,P=0.043;r=-0.619,P=0.028);HCC组患者PBMCs中IL-22mRNA表达水平与血清甲胎蛋白(AFP)水平呈明显正相关关系(r=0.664,P=0.000)。结论: 慢性乙肝重症化过程中,患者PBMCs中IL-22mRNA表达水平低下提示肝细胞再生障碍,且IL-22mRNA表达水平与TB一样能够反映患者病情严重程度。IL-22可能对HCC的发生发展起促进作用。

关键词: 白细胞介素22, 肝炎, 乙型, 慢性, 肝肿瘤

Abstract:

Objective: To detect the expression levels of IL-22 mRNA in peripheral blood mononuclear cells (PBMCs) of the patients with chronic hepatitis B (CHB),acute/subacute-on-chronic liver failure (severe hepatitis),and hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC),and to investigate their clinical significances. Methods: A total of 20 healthy controls (control group),33 patients with moderate CHB(moderate CHB group),21 patients with severe CHB(severe CHB group),16 patients with severe hepatitis(severe hepatitis group),16 patients with cirrhosis (cirrhosis group) , and 40 patients with HCC(HCC group) were enrolled.The expression levels of IL-22 mRNA in PBMCs of the subjects in various groups were tested by RT-PCR method;Pearson correlation analysis was used to analyze the relationship between the IL-22 mRNA expression levels and the important clinical indexes. Results: The expression levels of IL-22 mRNA in PBMCs of the patients in moderate CHB,severe CHB and cirrhosis groups were all higher than that in control group(P<0.001);there were no significant differences of the expression levels of IL-22 mRNA of the subjects between severe hepatitis group and control group (P=0.83);the expression level of IL-22 mRNA in PBMCs of the patients in HCC group was lower than that in control group (P<0.001);the expression level of IL-22 mRNA in PBMCs was negatively correlated with the serum total bilirubin(TB) level in severe CHB and severe hepatitis groups(r=-0.383,P=0.043;r=-0.619,P=0.028);the expression level of IL-22 mRNA was positively correlated with the serum alpha-fetoprotein (AFP) level in HCC group (r=-0.664,P=0.000). Conclusion: The decreasing of IL-22 mRNA expression level in the patients with deteriorative hepatitis suggests the dysfunction of liver regeneration;the IL-22 mRNA expression level,as TB,can reflect the severity of liver injury. IL-22 may have promotion effect on the occurrence and development of HCC.

Key words: interleukin-22, hepatitis B,chronic, carcinoma,hepatocellular

中图分类号: 

  • R512.62