特异性沉默α-catulin基因的表达对舌鳞状细胞癌TSCCA细胞增殖和凋亡的影响

doi:10.13481/j.1671-587x.20160313

摘要/Abstract

摘要：

目的: 探讨α-catulin基因对人舌鳞状细胞癌(TSCC)TSCCA细胞增殖和凋亡的影响,阐明α-catulin在TSCCA细胞生物学行为中的作用。方法: 取对数生长期的TSCCA细胞,分为α-catulin-siRNA转染组、空质粒转染组和空白对照组。RT-PCR法检测各组TSCCA细胞中α-catulinmRNA相对表达水平;Western blotting法检测各组TSCCA细胞中α-catulin蛋白的相对表达水平,MTT法检测细胞生长抑制率,流式细胞术检测各组TSCCA细胞凋亡率。结果: RT-PCR检测,与空质粒转染组和空白对照组比较,α-catulin-siRNA转染组TSCCA细胞中α-catulinmRNA相对表达水平降低(P<0.01),且随着时间的相对延长,α-catulinmRNA相对表达水平逐渐降低,72h时其相对表达水平低于24和48h时(P<0.01)。Western blotting法检测,与空质粒转染组和空白对照组比较,α-catulin-siRNA转染组TSCCA细胞中α-catulin蛋白相对表达水平降低(P<0.05)。MTT法检测,与空质粒转染组和空白对照组比较,α-catulin-siRNA转染组TSCCA细胞生长抑制率升高(P<0.001)。流式细胞术检测,与空质粒对照组和空白对照组比较,α-catulin-siRNA转染组TSCCA细胞凋亡率升高(P<0.001)。结论: 特异性沉默α-catulin基因的表达可抑制TSCC的TSCCA细胞的增殖,促进其凋亡。

关键词: 癌, 鳞状细胞, 舌肿瘤, &alpha, -catulin, TSCCA, 细胞增殖, 细胞凋亡

Abstract:

Objective: To explore the effects of silencing expression of α-catulin gene on the proliferation and apoptosis of human tongue squamous cell carcinoma(TSCC) TSCCA cells,and to clarify its role in the biological behavior of TSCCA cells. Methods: The TSCCA cells at logarithmic growth phase were selected and divided into α-catulin-siRNA transfection group,empty plasmid transfection group, and blank control group.The relative expression levels of α-catulin mRNA in the TSCCA cells in various groups were tested by RT-PCR method;the relative expression levels of α-catulin protein in TSCCA cells in various groups were detecetd by Western blotting method;the inhibitory rate of proliferation of TSCCA cells was checked by MTT method;The apoptotic rates of TSCCA cells were measured by flow cytometry(FCM). Results: The RT-PCR results showed that the relative expression level of α-catulin mRNA in the TSCCA cells in α-catulin-siRNA transfection group was lower than those in empty plasmid transfection group and blank control group(P<0.01); the relative expression levels of α-catulin mRNA were decreased gradually with the prolongation of time,and the relative expression level of α-catulin mRNA at 72 h was lower than those at 24 and 48 h(P<0.01).The Western blotting results revealed that compared with empty plasmid transfection group and blank control group,the relative expression level of α-catulin protein in the TSCCA cells in α-catulin-siRNA transfection group was decreased(P<0.05).The MTT results demonstrated that the inhibitory rate of growth of TSCCA cells in α-catulin-siRNA transfection group was higher than those in empty plasmid transfection group and blank control group(P<0.001).The FCM results exhibited that the apoptotic rate of TSCCA cells in α-catulin-siRNA transfection group was increased compared with empty plasmid transfection group and blank control group(P<0.001). Conclusion: Silencing the expression of α-catulin RNA specifically could suppress the growth of TSCCA cells and promote the apoptosis of TSCCA cells.

Key words: TSCCA, cell proliferation, apoptosis, carcinoma,squamous cell, tongueneoplasms, α-catulin

中图分类号:

R739.86

马雪, 张斌, 韩春耀, 刘明媛, 郝丽静, 葛树卿, 薛中原. 特异性沉默α-catulin基因的表达对舌鳞状细胞癌TSCCA细胞增殖和凋亡的影响[J]. 吉林大学学报(医学版), 2016, 42(03): 486-490.

MA Xue, ZHANG Bin, HAN Chunyao, LIU Mingyuan, HAO Lijing, GE Shuqing, XUE Zhongyuan. Effects of specific expression of α-catulin gene on proliferation and apoptosis of tongue squamous cell carcinoma TSCCA cells[J]. Journal of Jilin University Medicine Edition, 2016, 42(03): 486-490.

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