吉林大学学报(医学版) ›› 2020, Vol. 46 ›› Issue (02): 372-376.doi: 10.13481/j.1671-587x.20200227

• 临床研究 • 上一篇    

M1型巨噬细胞相关因子在重度慢性牙周炎患者牙龈组织中的表达及其意义

蔡如佳1, 关宁2, 刘乙臻1, 高秀秋1, 王琳源1   

  1. 1. 锦州医科大学附属第二医院牙周黏膜科, 辽宁 锦州 121000;
    2. 锦州医科大学附属第一医院脑与脊髓损伤重点实验室, 辽宁 锦州 121000
  • 收稿日期:2019-04-22 发布日期:2020-04-07
  • 通讯作者: 王琳源,副主任医师,硕士研究生导师(Tel:0416-2655132,E-mail:wanglinyuan2006@163.com) E-mail:wanglinyuan2006@163.com
  • 作者简介:蔡如佳(1993-),女,辽宁省凤城市人,在读医学硕士,主要从事牙周炎免疫调节方面的研究。
  • 基金资助:
    国家自然科学基金青年基金资助课题(81600870);辽宁省博士启动基金资助课题(201601360)

Expressions of M1 macrophage-related factors in gingiva tissue of patients with severe chronic periodontitis and their significances

CAI Rujia1, GUAN Ning2, LIU Yizhen1, GAO Xiuqiu1, WANG Linyuan1   

  1. 1. Department of Periodontics and Mucosa, Second Affiliated Hospital, Jinzhou Medical University, Jinzhou 121000, China;
    2. Key Laboratory of Brain and Spinal Cord Injury, First Affiliated Hospital, Jinzhou Medical University, Jinzhou 121000, China
  • Received:2019-04-22 Published:2020-04-07

摘要: 目的:探讨M1型巨噬细胞相关因子诱导型一氧化氮合酶(iNOS)和信号传导及转录激活因子1(STAT1)在重度慢性牙周炎患者牙龈组织中的表达情况,阐明M1型巨噬细胞在慢性牙周炎发生发展中的可能作用。方法:选择15例重度慢性牙周炎患者的病损牙龈组织作为实验组,15例需要拔除第三磨牙患者的健康牙龈组织作为对照组。采用RT-PCR法检测2组患者牙龈组织中iNOS和STAT1 mRNA表达水平,Western blotting法检测2组患者牙龈组织中iNOS和STAT1蛋白表达水平。结果:与对照组比较,实验组患者牙龈组织中iNOS和STAT1 mRNA表达水平明显升高(P<0.01);与对照组比较,实验组患者牙龈组织中iNOS和STAT1蛋白表达水平明显升高(P<0.01)。结论:在重度慢性牙周炎中M1型巨噬细胞介导的免疫应答增强,表明M1型巨噬细胞参与牙周组织的炎症反应和组织损伤。

关键词: M1型巨噬细胞, 慢性牙周炎, 诱导型一氧化氮合酶, 信号传导及转录激活因子1

Abstract: Objective: To investigate the expressions of M1 macrophage-related factors induced nitric oxide synthase(iNOS) and signal transducers and activators of transcription 1(STAT1) in gingiva tissue of the patients with severe chronic periodontitis, and to elucidate the possible role of M1 macrophages in the occurrence and development of chronic periodontitis. Methods: The gingiva tissue of 15 patients with severe chronic periodontitis was selected as experimental group, and 15 cases of healthy gingiva tissue of the patients requiring removal of the third molars were used as control group.The expression levels of iNOS and STAT1 mRNA in the gingiva tissues of the patients in two groups were detected by RT-PCR.The expression levels of iNOS and STAT1 proteins in the gingiva tissues of the patients in two groups were detected by Western blotting method. Results: Compared with control group,the expression levels of iNOS and STAT1 mRNA in the gingiva tissue of the patients in experimental group were significantly increased (P<0.01);compared with control group,the expression levels of iNOS and STAT1 proteins in the gingiva tissue of the patients in experiment group were also significantly increased (P<0.01). Conclusion: M1 macrophage-mediated immune response is enhanced in severe chronic periodontitis, suggesting that M1 macrophages are involved in the inflammatory response and tissue damage of periodontal tissue.

Key words: M1 macrophage, chronic periodontitis, induced nitric oxide synthase, signal transducers and activators of transcription 1

中图分类号: 

  • R781.42