miR-107对卵巢癌细胞免疫逃逸的调控和紫杉醇耐药性的影响

doi:10.13481/j.1671-587X.20220323

摘要/Abstract

摘要： 目的

探讨微小RNA-107（miR-107）通过丝裂原活化蛋白3激酶7（MAP3K7）调控卵巢癌细胞免疫逃逸对紫杉醇（TAX）耐药性的影响，并阐明其作用机制。

方法

293T细胞分为miR-NC组（转染miR-NC）和miR-107 mimics组（转染miR-107 mimics）。采用浓度梯度递增法处理卵巢癌SKOV3细胞，获得TAX耐药细胞SKOV3/TAX。SKOV3/TAX细胞随机分为空白对照组、TAX（给予4.0 μmol·L^－1TAX）组、TAX+miR-107 mimics（给予4.0 μmol·L^－1TAX且转染miR-107 mimics）组和TAX+miR-107 mimics+pcDNA-MAP3K7（给予4.0 μmol·L^－1 TAX且转染miR-107 mimics和pcDNA-MAP3K7）组；分别与人外周血淋巴细胞HPBL共培养，分为HPBL组、HPBL+SKOV3/TAX组、HPBL+SKOV3/TAX+TAX组、HPBL+SKOV3/TAX+TAX+miR-107 mimics组和HPBL+SKOV3/TAX+TAX+miR-107 mimics+pcDNA-MAP3K7组。实时荧光定量PCR（RT-qPCR）法检测细胞中miR-107表达水平，双荧光素酶报告基因实验验证miR-107和MAP3K7的靶向关系，Western blotting法检测各组细胞中MAP3K7和FAS蛋白表达水平，CCK-8法检测各组细胞存活率，流式细胞术检测各组细胞凋亡率。

结果

与人卵巢上皮细胞HOSEpic比较，卵巢癌细胞中miR-107表达水平降低（P<0.05）。MAP3K7的3′-UTR与miR-107存在靶向结合位点。双荧光素酶报告基因实验，与miR-NC组比较，过表达miR-107组野生型MAP3K7的荧光素酶活性降低（P<0.01）。Western blotting法检测，过表达miR-107组细胞中MAP3K7蛋白表达水平低于miR-NC组（P<0.01）。RT-qPCR检测，与空白对照组比较，TAX组SKOV3/TAX细胞中miR-107 mRNA表达水平升高（P<0.01）；与TAX组比较，TAX+miR-107 mimics组SKOV3/TAX细胞中miR-107 mRNA表达水平升高（P<0.05）。与空白对照组比较，TAX组SKOV3/TAX细胞中MAP3K7和FAS蛋白表达水平降低（P<0.01）；与TAX组比较，TAX+miR-107 mimics 组SKOV3/TAX细胞中MAP3K7和FAS蛋白表达水平降低（P<0.05或P<0.01）；与TAX+miR-107 mimics组比较，TAX+miR-107 mimics+pcDNA-MAP3K7组SKOV3/TAX细胞中MAP3K7和FAS蛋白表达水平升高（P<0.05或P<0.01）。与HPBL+SKOV3/TAX组比较， HPBL+SKOV3/TAX+TAX组HPBL细胞中FAS蛋白表达水平降低（P<0.05），细胞凋亡率降低（P<0.05）；与HPBL+SKOV3/TAX+TAX组比较，HPBL+SKOV3/TAX+TAX+miR-107 mimics组HPBL细胞中FAS蛋白表达水平和细胞凋亡率降低（P<0.05）；与HPBL+SKOV3/TAX+TAX+miR-107 mimics 组比较，HPBL+SKOV3/TAX+TAX+miR-107 mimics+ pcDNA-MAP3K7组HPBL细胞中FAS蛋白表达水平降低（P<0.01），且细胞凋亡率降低（P<0.01）。与空白对照组比较， TAX组SKOV3/TAX细胞存活率降低（P<0.05）；与TAX组比较，TAX+miR-107 mimics组SKOV3/TAX细胞存活率降低（P<0.05）。与TAX+miR-107 mimics组比较，TAX+miR-107mimics+pcDNA-MAP3K7组SKOV3/TAX细胞存活率明显升高（P<0.05）。与空白对照组比较，TAX组SKOV3/TAX细胞凋亡率明显升高（P<0.05）；与TAX组比较，TAX+ miR-107组SKOV3/TAX细胞凋亡率升高（P<0.05）；与TAX+miR-107 mimics组比较，TAX+miR-107 mimics+pcDNA-MAP3K7组细胞凋亡率降低（P<0.05）。

结论

miR-107在SKOV3/TAX细胞中呈低表达，且miR-107通过抑制MAP3K7的表达，抑制SKOV3/TAX细胞免疫逃逸，缓解卵巢癌细胞TAX耐药性。

关键词: 卵巢肿瘤, 微小RNA-107, 免疫逃逸, 紫杉醇, 耐药

Abstract:

Objective： To investigate the effect of microRNA-107（miR-107）on the taxol （TAX）-resistance in the ovarian cancer cells by regulating immune escape through the mitogen-activated protein 3 kinase 7 （MAP3K7），and to charify its mechanism.

Methods

The 293T cells were divided into miR-NC group（transfected with miR-NC） and miR-107 mimics group（transfected with miR-107 mimics）.The ovarian cancer SKOV3 cells were treated with concentration gradient increasing method to obtain the TAX resistant SKOV3/TAX cells. The SKOV3/TAX cells were randomly divided into blank control group， TAX （given 4.0 μmol·L^－1 TAX） group， TAX+miR-107 mimics （given 4.0 μmol·L^－1 TAX and transfected with miR-107 mimics） group，TAX+miR-107 mimics+pcDNA-MAP3K7 （given 4.0 μ mol·L^－1 TAX and transfected with miR-107 mimics and pcDNA-MAP3K7）， and co-cultured with the human peripheral blood lymphocytes HPBL， respectively. Then they were divided into HPBL group， HPBL+SKOV3/TAX group， HPBL+ SKOV3/TAX+TAX group， HPBL + SKOV3/TAX+TAX+miR-107 mimics group and HPBL+ SKOV3/TAX+TAX+miR-107 mimics+pcDNA-MAP3K7 group. The expression levels of miR-107 in the cells in various groups were detected by real-time fluorescence quantitative PCR （RT-qPCR）method， the targeting relationship between miR-107 and MAP3K7 was verified by double luciferase reporter gene experiment， the expression levels of MAP3K7 and FAS proteins in the cells in various groups were detected by Western blotting method， the survival rates of the cells in various groups were detected by CCK-8 method， and the apoptotic rates of the cells in various groups were detected by flow cytometry.

Results

Compared with the human ovarian epithelial cells HOSEpic， the expression level of miR-107 in the ovarian cancer cells was decreased （P<0.05）. There was a targeted binding site between 3′-UTR of MAP3K7 and miR-107. The double luciferase reporter gene experiment results showed that compared with miR-NC group， the luciferase activity of wild-type MAP3K7 in miR-107 over-expression group was decreased （P<0.01）. The Western blotting results showed that the expression level of MAP3K7 protein in the cells in miR-107 over-expression group was significantly lower than that in miR-NC group （P<0.01）. The RT-qPCR results showed that compared with blank control group， the expression level of miR-107 mRNA in the SKOV3/TAX cells in TAX group was increased（P<0.01）； compared with TAX group， the expression level of miR-107 mRNA in the SKOV3/TAX cells in miR-107 MICs+TAX group was increased（P<0.05）. Compared with blank control group， the expression levels of MAP3K7 and FAS proteins in the SKOV3 / TAX cells in TAX group were decreased （P<0.01）； compared with TAX group， the expression levels of MAP3K7 and FAS proteins in the cells in TAX+miR-107 mimics SKOV3/TAX group were decreased （P<0.05 or P<0.01）； compared with TAX+miR-107 mimics group， the expression levels of MAP3K7 and FAS proteins in the SKOV3/TAX cells in TAX+miR-107 mimics+pcDNA-MAP3K7 group were increased（P<0.05 or P<0.01）.Compared with HPBL+SKOV3/TAX group， the expression level of FAS protein and apoptotic rate of the HPBL cells in HPBL+SKOV3/TAX+TAX group were decreased （P<0.05）； compared with HPBL+SKOV3/TAX+TAX group， the expression level of FAS protein and the apoptotic rate in the HPBL cells in HPBL+SKOV3/TAX+TAX + miR-107 mimics group were decreased（P<0.05）； compared with HPBL+SKOV3/TAX+TAX+miR-107 mimics group， the expression level of FAS protein in the HPBL cells in HPBL+SKOV3/TAX+TAX+miR-107 mimics+pcDNA-MAP3K7 group was decreased （P<0.01）， and the apoptotic rate was decreased （P<0.01）. Compared with blank control group， the survival rate of the SKOV3/TAX cells in TAX group was decreased （P<0.05）；compared with TAX group，the survival rate of the SKOV3/TAX cells in TAX+miR-107 mimics group was significantly decreased（P<0.05）. Compared with TAX + miR-107 mimics group， the survival rate of the SKOV3/TAX cells in TAX+miR-107 mimics+pcDNA-MAP3K7 group was increased （P<0.05）. The apoptotic rate of the SKOV3/TAX cells in TAX group was significantly higher than that in blank control group （P<0.05）， the apoptotic rate of the SKOV3/TAX cells in TAX+miR-107 group was significantly higher than that in TAX group （P<0.05）， and the apoptotic rate of the cells in TAX+miR-107 mimics+pcDNA-MAP3K7 group was lower than that in TAX+miR-107 mimics group （P<0.05）.

Conclusion

MiR-107 is low-expressed in the SKOV3/TAX cells， and over-expression of miR-107 could inhibit the immune escape of the SKOV3/TAX cells and alleviate the TAX resistance of the ovarian cancer cells by inhibiting the expression of MAP3K7.

Key words: Ovarian neoplasm, MicroRNA-107, Immune escape, Taxol, Drug resistance

中图分类号:

R737.31

王青慧,李波,胡传翠,聂明朝,郑小妹. miR-107对卵巢癌细胞免疫逃逸的调控和紫杉醇耐药性的影响[J]. 吉林大学学报(医学版), 2022, 48(3): 734-743.

Qinghui WANG,Bo LI,Chuancui HU,Mingchao NIE,Xiaomei ZHENG. Effects of miR-107 on regulation of immune escape and taxol resistance of ovarian cancer cells[J]. Journal of Jilin University(Medicine Edition), 2022, 48(3): 734-743.

图/表 12

图1

图2

图3

表1

表2

图4

表3

图5

图6

表4

图7

表5

参考文献 28

1	STEWART C， RALYEA C， LOCKWOOD S. Ovarian cancer： an integrated review［J］. Semin Oncol Nurs， 2019， 35（2）： 151-156.
2	MCMULLEN M， KARAKASIS K， MADARIAGA A， et al. Overcoming platinum and PARP-inhibitor resistance in ovarian cancer［J］. Cancers， 2020， 12（6）： 1607.
3	LAU T S， CHAN L K Y， MAN G C W， et al. Paclitaxel induces immunogenic cell death in ovarian cancer via TLR4/IKK₂/SNARE-dependent exocytosis［J］.Cancer Immunol Res，2020，8（8）： 1099-1111.
4	WOLF D， FIEGL H， ZEIMET A G， et al. High RIG-I expression in ovarian cancer associates with an immune-escape signature and poor clinical outcome［J］. Int J Cancer， 2020， 146（7）： 2007-2018.
5	DIECKMANN K P， RADTKE A， GECZI L， et al. Serum levels of microRNA-371a-3p （M371 test） as a new biomarker of testicular germ cell tumors： results of a prospective multicentric study［J］. J Clin Oncol， 2019， 37（16）： 1412-1423.
6	XIONG Y Q， LIU Z M， LI Z， et al. Long non‑coding RNA nuclear paraspeckle assembly transcript 1 interacts with microRNA‑107 to modulate breast cancer growth and metastasis by targeting carnitine palmitoyltransferase‑1［J］. Int J Oncol， 2019， 55（5）： 1125-1136.
7	CHEN H A， LI C C， LIN Y J， et al. Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells［J］. Aging， 2021， 13（8）： 12046-12057.
8	SU H W， TAO T， YANG Z， et al. Circular RNA cTFRC acts as the sponge of microRNA-107 to promote bladder carcinoma progression［J］. Mol Cancer， 2019， 18（1）： 27.
9	TANG Z H， FANG Y X， DU R. microRNA-107 induces cell cycle arrests by directly targeting cyclin E1 in ovarian cancer［J］. Biochem Biophys Res Commun， 2019， 512（2）： 331-337.
10	LHEUREUX S， BRAUNSTEIN M， OZA A M. Epithelial ovarian cancer： evolution of management in the era of precision medicine［J］. CA Cancer J Clin， 2019， 69（4）： 280-304.
11	UPADHYAY R， BOIARSKY J A， PANTSULAIA G， et al. A critical role for fas-mediated off-target tumor killing in T-cell immunotherapy［J］. Cancer Discov， 2021， 11（3）： 599-613.
12	YAMADA A， ARAKAKI R， SAITO M， et al. Dual role of fas/FasL-mediated signal in peripheral immune tolerance［J］. Front Immunol， 2017， 8： 403.
13	PARDOLL D M. The blockade of immune checkpoints in cancer immunotherapy［J］. Nat Rev Cancer， 2012， 12（4）： 252-264.
14	IQBAL M A， ARORA S， PRAKASAM G， et al. MicroRNA in lung cancer： role， mechanisms， pathways and therapeutic relevance［J］. Mol Aspects Med， 2019， 70： 3-20.
15	PERRIER A， DIDELOT A， LAURENT-PUIG P，et al.Epigenetic mechanisms of resistance to immune checkpoint inhibitors［J］. Biomolecules， 2020， 10（7）： 1061.
16	FUJITA Y， YAGISHITA S， HAGIWARA K， et al. The clinical relevance of the miR-197/CKS1B/STAT3-mediated PD-L1 network in chemoresistant non-small-cell lung cancer［J］. Mol Ther， 2015， 23（4）： 717-727.
17	NEVIANI P， WISE P M， MURTADHA M， et al. Natural killer-derived exosomal miR-186 inhibits neuroblastoma growth and immune escape mechanisms［J］. Cancer Res， 2019， 79（6）： 1151-1164.
18	JIN D， GUO J W， WU Y， et al. M 6 A demethylase ALKBH₅ inhibits tumor growth and metastasis by reducing YTHDFs-mediated YAP expression and inhibiting miR-107/LATS2-mediated YAP activity in NSCLC［J］. Mol Cancer， 2020， 19（1）： 40.
19	WEI J， XU H F， WEI W， et al. circHIPK₃ promotes cell proliferation and migration of gastric cancer by sponging miR-107 and regulating BDNF expression［J］. Onco Targets Ther， 2020， 13： 1613-1624.
20	TIAN X H， WU Y Y， YANG Y T， et al. Long noncoding RNA LINC00662 promotes M2 macrophage polarization and hepatocellular carcinoma progression via activating Wnt/β-catenin signaling［J］. Mol Oncol， 2020， 14（2）： 462-483.
21	FU Y X， LIN L W， XIA L G. miR-107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1［J］. Cell Mol Biol Lett， 2019， 24： 31.
22	WEI X Q， LEI Y J， LI M J， et al. miR-107 inhibited malignant biological behavior of non-small cell lung cancer cells by regulating the STK33/ERK signaling pathway in vivo and vitro ［J］.J Thorac Dis， 2020，12（4）： 1540-1551.
23	CORDAS DOS SANTOS D M， EILERS J， SOSA VIZCAINO A， et al. MAP3K7 is recurrently deleted in pediatric T-lymphoblastic leukemia and affects cell proliferation independently of NF-κB［J］. BMC Cancer， 2018， 18（1）： 663.
24	LE GOFF C， ROGERS C， LE GOFF W， et al. Heterozygous mutations in MAP3K7， encoding TGF-β-activated kinase 1， cause cardiospondylocarpofacial syndrome［J］. Am J Hum Genet， 2016，99（2）： 407-413.
25	ZHOU L L， DONG J L， HUANG G， et al. microRNA-143 inhibits cell growth by targeting ERK5 and MAP3K7 in breast cancer［J］. Braz J Med Biol Res， 2017， 50（8）： e5891.
26	FAN H， GE Y G， MA X， et al. Long non-coding RNA CCDC144NL-AS1 sponges miR-143-3p and regulates MAP3K7 by acting as a competing endogenous RNA in gastric cancer［J］. Cell Death Dis， 2020， 11（7）： 521.
27	ZEHAVI L， SCHAYEK H， JACOB-HIRSCH J， et al. miR-377 targets E2F3 and alters the NF-kB signaling pathway through MAP3K7 in malignant melanoma［J］. Mol Cancer， 2015， 14： 68.
28	JIN X， DING D L， YAN Y Q， et al. Phosphorylated RB promotes cancer immunity by inhibiting NF-κB activation and PD-L1 expression［J］. Mol Cell， 2019， 73（1）： 22-35.e6.

Group	Survival rate
TAX (μmol·L^－1)
0	99.21±0.48
1	84.29±2.78
2	65.07±3.54
3	52.86±3.09
4	44.93±3.29
5	32.52±3.11

Group	Expression level of miR-107 mRNA
Blank control	99.21±0.48
TAX	84.29±2.78^*
TAX+miR-107 mimics	65.07±3.54^△
TAX+miR-107 mimics+pcDNA-MAP3K7	52.86±3.09

Group	Expression level of MAP3K7	Expression level of FAS
Blank control	1.01±0.12	1.00±0.07
TAX	0.56±0.10^*	0.74±0.08^*
TAX+miR-107 mimics	0.25±0.07^△	0.30±0.06^△△
TAX+miR-107 mimics+pcDNA-MAP3K7	0.68±0.05^#	0.59±0.10^#

Group	Expression level of FAS	Apoptotic rate(η/%)
HPBL	1.02±0.11	6.24±1.77
HPBL+SKOV3/TAX	2.58±0.30^*	30.45±4.98^*
HPBL+SKOV3/TAX+TAX	1.95±0.19^△	20.33±2.95^△△
HPBL+SKOV3/TAX+TAX+miR-107 mimics	1.54±0.10^#	11.15±2.18^#
HPBL+SKOV3/TAX+TAX+miR-107 mimics+ pcDNA-MAP3K7	2.04±0.19^○	21.00±3.00^○

Group	Survival rate	Apoptotic rate
Blank control	1.95±0.22	11.01±2.17
TAX	1.56±0.08^*	17.43±1.68^**
TAX+miR-107 mimics	1.07±0.17^△	26.42±3.03^△
TAX+miR-107 mimics+pcDNA-MAP3K7	1.47±0.12^#	20.51±0.95^#