关键词: 脑, 细胞低氧, 神经元, 一氧化氮合酶, 生物合成, 小鼠

Abstract: Objective To explore the mechanism of proconditioning to hypoxia. Methods The changes of NOS neuron of mouse brain in proconditioning to hypoxia were detected by histochemistry method. Results Compared with control mice, the number, colour, shape, dendrites and axons number and length of NOS positive neurons in cerebral cortex was not changed after mice were exposed to hypoxia for one time, but the dendrites and axons of NOS neuron became thicker. Compared with control mice, NOS neurons in cerebral cortex was not changed after mice were exposed to hypoxia for four times. There were little NOS neurons in hippocampus of control mice and NOS neurons were light positive. Compared with control mice, the number of NOS positive neurons in hippocampus increased and NOS neuron became strong positive after mice were exposed to hypoxia for one time. Compared with mice exposed to hypoxia for one time, the number of NOS positive neurons in hippocampus decreased and their colour became light after mice were exposed to hypoxia for four times. Conclusion The changes of NOS neurons can enhance generation of proconditioning to hypoxia.

Key words: brain, cell hypoxia, neurons, nitric-oxide synthase, biosynthesis, mice