关键词: 壳聚糖, 微囊, 帕金森病, 治疗, 细胞移植, 疾病模型, 动物

Abstract: Objective To investigate the immunoisolation effect and biocompatibility of sodium alginate/chitosan. Methods The alginate-chitosan-alginate (ACA) was used as the microcapsule.Encapsulated PC12 cells，PC12 cells and empty microcapsule were transplanted respectively into the striatum of the Parkinsonian rats.The behavioral changes were tested by apomorphine.The morphological alterations were observed by striatal HE stain. The retrieved encapsulated PC12 cells were recultured and their activities were tested by MTT and Trypan blue test. Results The encapsulated PC12 cells could improve the rotational behavior of Parkinson′s disease (PD) model rats and there was a significant difference (P<0.05) comparing with pretransplantation group and empty capsule transplantation group. There was no disruption and no tumor formation in this group, and the inflammatory reaction around the capsule was slight. Although the unencapsulated PC12 cells could also improve the rotational behavior,the symptom improvement lasted only two months.In addition,several of the grafts developed into mortal tumor. The well growth and the good biological activities of the retrieved PC12 cells were proved using the MTT and Trypan blue test. Conclusion Sodium alginte/chitosan has a good biocompatibility. ACA microcapsule can both isolate the host immune system effectively and prevent the hypersegmentation of the cells inside the capsule.

Key words: chitosan, microcapsule, Parkinson disease, therapy, transplantation, disease models, animal