关键词: 组织学前列腺炎, 单核苷酸多态性, 维生素D受体

Abstract: Abstract:Objective To investigate the association between single nucleotide polymorphism (SNP) of Fok Ⅰ in vitamin D receptor (VDR) gene and different degrees of histological prostatitis(HP),and to discuss the role of genetic factors in  the onset of HP.Methods  169 patients with benign prostatic hyperplasia (BPH) complicated with  HP were selected.The mild(79 cases),moderate(52 cases) and  severe(38 cases) HP patients were distinguished by pathological  examination.Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to determine the  SNP of Fok Ⅰ of patients.The distribution of genotypic and allelic frequencies of the patients in various groups was analyzed.Results The  distribution of genotypic frequency of Fok Ⅰ site in  mild and moderate  HP patients didn’t deviate from  Hardy-Weinberg equilibrium(P>0.05).The  distribution of genotypic frequency of Fok Ⅰ gene in  severe HP patients deviated from  Hardy-Weinberg equilibrium(P<0.05).The  distribution of FF,Ff,ff genotypes  in  mild and moderate HP patients were 32%（25/79）,46%（36/79）;22%（18/79）,23%（12/52）;40%（21/52），37%（19/52）, respectively;there was no significant difference between two groups(P>0.05).The  allelic frequencies of F and f were 54%（86/158），46%（72/158） in  mild HP patients  and 43%（45/104），57%（59/104） in moderate  HP patients, respectively;there was no significant difference between two groups (P>0.05).Conclusion VDR gene FokⅠ SNP may not associate with the  occurrence of different degrees of HP.

Key words: histological prostatitis, single nucleotide polymorphism, vitamin D receptor