冬凌草甲素对子宫内膜癌HEC-1B细胞增殖、迁移和侵袭及lncRNA CCAT1表达的影响

doi:10.13481/j.1671-587X.20210218

Abstract

Abstract: Objective

To investigate the effects of oridonin on the proliferation， migration and invasion of endometrial cancer HEC-1B cells， and to elucidate its possible mechanism.

Methods

The endometrial cancer HEC-1B cells in logarithmic growth phase were divided into control group（DMSO）， positive control group（2.5 mg·L^－1cisplatin），low dose（10 μmol·L^－1）of oridonin group， medium dose （20 μmol·L^－1）of oridonin group and high dose （40 μmol·L^－1）of oridonin group. MTT assay was used to detect the inhibitory rates of proliferation of cells. Flow cytometry was used to determine the apoptotic rates of HEC-1B cells. Cell scratch test was used to detect the cell migration ability. Transwell chamber assay was used to detect the invasion ability of HEC-1B cells. Real-time fluorescence quantitative PCR（RT-qPCR） was used to detect the expression levels of lncRNA CCAT1 in the cells.

Results

Compared with control group， the apoptotic rates of HEC-1B cells in positive control group， low，medium and high doses of oridonin groups were significantly increased （P<0.05），the scratch healing rates and the number of transmembrane cells were significantly reduced （P<0.05），and the expression levels of lncRNA CCAT1 in the HEC-1B cells were significantly decreased （P<0.05）. Compared with low dose of oridonin group， the apoptotic rates of HEC-1B cells in medium and high doses of oridonin groups were significantly increased （P<0.05），the scratch healing rates and the number of transmembrane cells were significantly reduced （P<0.05），and the expression levels of lncRNA CCAT1 in the HEC-1B cells were significantly decreased （P<0.05）. Compared with medium dose of oridonin group， the apoptotic rate of HEC-1B cells in high dose of oridonin group was significantly increased （P<0.05），the scratch healing rate and the number of transmembrane cells were significantly reduced （P<0.05），and the expression level of lncRNA CCAT1 in the HEC-1B cells was significantly decreased （P<0.05）.

Conclusion

Oridonin can inhibit the proliferation， migration，and invasion and promote the apoptosis of endometrial cancer HEC-1B cells， and its mechanism may be related to down-regulating the expression level of lncRNA CCAT1.

Key words: oridonin, lncRNA CCAT1, endometrial cancer, cell migration, cell invasion

CLC Number:

R737.33

Jing WANG,Jing LIU,Xujing WEI,Yafei DU,Guiying FANG,Lin LI. Effects of oridonin on proliferation, migration, invasion and expression of lncRNA CCAT1 of endometrial carcinoma HEC-1B cells[J].Journal of Jilin University(Medicine Edition), 2021, 47(2): 384-389.

Figures/Tables 5

Tab. 1

Fig. 1

Tab.2

Fig. 2

Tab. 3

References 22

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Group	Inhibitory rate of proliferation	Apoptotic rate
Control	—	4.57±0.38
Positive control Oridonin(μmol·L^－1)	51.20±4.17	54.71±3.44^*
Low dose	18.41±2.17	35.46±1.05^*
Medium dose	32.01±3.56^△	40.55±3.10^*△
High dose	45.24±3.82^△#	44.82±2.31^*△#

Group	Scatch healing rate（η/%）	Number of transmembrane cells(n)
Control	84.3±7.7	196±25
Positive control Oridonin(μmol·L^－1)	34.8±5.3^*	58±16^*
Low dose	66.4±7.1^*	121±10^*
Medium dose	51.6±6.1^*△	99±15^*△
High dose	37.3±5.2^*△#	74±11^*△#

Group	lncRNA CCAT1
Control	1.13±0.24
Positive control Oridonin(μmol·L^－1)	0.96±0.15
Low dose	0.66±0.13^*
Medium dose	0.47±0.08^△*
High dose	0.32±0.10^*△#

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Effects of oridonin on proliferation, migration, invasion and expression of lncRNA CCAT1 of endometrial carcinoma HEC-1B cells

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