Abstract:

Objective To detect the expressions of Cks1,Skp2, and p27^kip1 proteins in tongue squamous cell carcinoma tissue and to analyze their associations with the clinicopathological characteristics of the patients with tongue squamous cell carcinoma, and to clarify their roles in the development and prognosis of tongue squamous cell carcinoma.Methods The expressions of Cks1,Skp2 and p27^kip1 proteins in 45 cases of human tongue squamous cell carcinoma tissue,30 cases of adjacent carcinoma tissue and 10 cases of normal tongue mucosa tissue were detected by immunohistochemistry;the differences of the positive expression rates of Cks1,Skp2,and p27^kip1 proteins in different kinds of tongue tissues were compared; the relationships between the expressions of Cks1,Skp2 and p27^kip1 proteins with the clinicopathological parameters of the patients were analyzed.Results The expression levels of Cks1 and Skp2 proteins in tongue carcinoma tissue were obviously increased and the positive expression rate of Cks1 and Skp2 proteins were 73.3% and 62.2%.The expression levels of Cks1 and Skp2 proteins in the adjacent carcinoma tissue and normal tongue mucosa tissue were decreased gradually,and the positive expression rates of Cks1 and Skp2 proteins were 23.3%,10.0% and 36.7%,10.0%, respectively.The expression level of p27^kip1 protein in the tongue carcinoma tissue was decreased or loss, and the positive expression rate of p27^kip1 protein was 24.4%.The expression levels of p27^kip1 proten in the adjacent carcinoma tissue and normal tongue mucosa tissue were increased gradually,and the positive expression rates of p27^kip1 protein were 30.0% and 70.0%,respectively.The postive expression rates of Cks1 and Skp2 proteins in the tongue carcinoma tissue were higher than those in the adjacent carcinoma tissue and normal tongue mucosa tissue(P<0.05),and the positive expression rate of p27^kip1 protein was lower than those in the adjacent carcinoma tissue and normal tongue mucosa tissue (P<0.05).The positive expression rates of Cks1,Skp2 and p27^kip1 proteins in the tongue carcinoma tissue were significantly correlated with the lymph node metastasis,TNM stages,differentiation degrees,and clinical stages(P<0.05);but they were not correlated with gender and age (P>0.05).Conclusion The positive expression rates of Cks1,Skp2 proteins in tongue carcinoma tissue are increased and the positive expression rate of p27^kip1 protein is decreased;the detection of the positive expression rates of Cks1,Skp2 and p27^kip1 proteins contributes to judging the malignancy grade and prognosis of the tongue sequamous cell carcinoma.

Key words: cyclin dependent kinase subunit 1, S-phase kinase associated protein 2, p27^kip1 protein, tongue squamous cell carcinoma