Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (04): 706-710.doi: 10.13481/j.1671-587x.20150407

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Influence of estrogen receptor-alpha promoter methylation status in expression levels of MTA1 gene and protein in breast cancer cell lines

SUN Yanan1, MAO Xiaoyun2, LIU Chong2, CHEN Hao2, GUO Yang2, JIN Feng2   

  1. 1. Research Unit of General Surgery, Department of General Surgery & Burn Ward, First Affiliated Hospital, China Medical University, Shenyang 110001, China;
    2. Research Unit of General Surgery, Department of Breast Surgery, First Affiliated Hospital, China Medical University, Shenyang 110001, China
  • Received:2015-03-26 Published:2015-08-01

Abstract:

Objective To discuss the influence of the changes of estrogen receptor-alpha (ERα) promoter methylation in the expression levels of MTA1 mRNA and protein in breast cancer cell lines,and to illustrate the effect of ERα promoter on the expression of MTA1 gene after demethylation. Methods The breast cancer cell lines MDA-MB-435s in ERα promoter in hypermethylation status and MDA-MB-231 in ERα promoter in hypomethylation status were treated with 5-Aza-2'-deoxycytidine (5-Aza-dC) (5-Aza-dc treated groups),while the two kinds of cell lines were without 5-Aza-dC treatment were used as untreated groups.The methylation status of ERα promoter of the cells in various groups was detected by methylation-specific PCR (MSP).The expression levels of MTA1 mRNA and protein in MDA-MB-435s and MDA-MB-231 cells in various groups were detected with RT-PCR and Western blotting method. Results Both in the MDA-MB-435s and the MDA-MB-231 cells,the methylation status of ERα was modified from methylation to unmethylation by being treated with 5-Aza-dC.Furthermore,both in the MDA-MB-435s and the MDA-MB-231,compared with untreated groups,the expression levels of MTA1 mRNA and protein in 5-Aza-dC treated groups were down-regulated (P<0.05). Conclusion After the methylation status of ERα promoter is modified from methylation to unmethylation in breast cancer cell lines MDA-MB-435s and MDA-MB-231,the expression levels the MTA1 mRNA and protein are down-regulated.It's illustrated the expression of MTA1 can be lowered after ERα promoter demethylation,and the results further verify the regulation of the relationship between ERα methylation and MTA1 gene.

Key words: metastasis-associated gene 1, estrogen receptor-alpha, methylation, epigenetic, breast neoplasms

CLC Number: 

  • R737.9