紫薯花青素抗结肠癌作用及其诱导细胞凋亡机制

doi:10.13481/j.1671-587x.20150422

Abstract

Abstract:

Objective To explore the cytotoxicity of anthocyanins from purple sweet potato on the human colorectal carcinoma cells (HCT-116),and to illuminate the mechanism of inducing apoptosis. Methods The HCT-116 cells and fibroblasts HF-91 at the logarithmic phase were divided intocontrol group(PBS),positive drug group (25 mg·L^-1 DDP),PCI groups (final concentrations:25,50,and 100 mg·L^-1). The inhibitroy rates of cell proliferation were detected with CCK8 assay;the apoptosis was observed using AO/EB method;the JC-1 mitochondrial membrane potential was detected by flow cytometry; the expression levels of p53 and caspase-3 mRNA were measured by qPCR method. Results Compared with DDP group,the inhibitory rate of proliferation of HCT-116 cells in 100 mg·L^-1 PCI group was increased(P<0.05),and the inhibitory rates of proliferation of HF-91 cells in different concentraitons of PCI groups were decreased(P<0.05) in a concentration-dependent manner.Compared with DDP group,the apoptotic rates of HCT-116 cells in 50 and 100 mg·L^-1 PCI groups were increased(P<0.05).Compared with control group,the expression levels of p53 and caspase-3 mRNA in HCT-116 cells in DDP group and different concentrations of PCI groups were increased(P<0.05).Compared with DDP group,the expression levels of caspase-3 mRNA in different concentrations of PCI groups were significantly increased(P<0.05),and the expression level of p53 mRNA in 100 mg·L^-1 PCI group was obviously increased(P<0.05). Conclusion Proanthocyanidin has strong cytotoxicity on the digestive system tumor cells,and has little cytotoxicity on the normal cells.

Key words: purple sweet potato, anthocyanins, colonic neoplasms, apoptosis

CLC Number:

R735.3

CHEN Weili, ZHANG Chen, ZHANG Xuhao, LIU Dongying, ZHAO Xi. Inhibitory effect of proanthocyanidins on human colorectal carcinoma and its apoptosis-induction mechanisms[J].Journal of Jilin University Medicine Edition, 2015, 41(04): 785-789.

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Inhibitory effect of proanthocyanidins on human colorectal carcinoma and its apoptosis-induction mechanisms

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