Abstract:

Objective To observe the induction effect of myricetin on the apoptosis and the inhibitory effect on the growth of the human ovarian cancer SKOV3 cells,and to explore the probable mechanisms. Methods The human ovarian cancer SKOV3 cells were cultured in vitro.The cells were divided into control group,low,middle and high concentrations of myricetin groups,which were treated with different concentrations (0,20,40 and 60 mg·L^-1) of myricetin.Then the survival rates of cells were tested by MTT method;Laser scanning confocal microscope was used to observe the morphological changes,and the rates of abnormal necleuses were calculated.The expressions of active Caspase-3 in the SKOV3 cells in various groups were observed by immunofluorescence method.The expression levels of γ-H₂AX in the SKOV3 cells in various groups were detected by Western blotting method. Results Compared with control group,the survival rates of the SKOV3 cells in myricetin groups were significantly decreased(t=-12.35,t=-11.42,t=-10.21,P<0.05).The morphology of nucleuses of SKOV3 cells in various groups had some changes for the cause of apoptosis which were observed by laser scanning confocal microscope,nuclear shrinkage for example.The rates of abnormal nucleuses in low,middle and high concentration of myricetin groups were obviously higher than that in control group(t=6.87,t=9.35,t=7.22,P<0.05),and the rate of abnormal nucleus in high concentration of myricetin group was higher than those in low and middle concentrations of myricetin groups(t=9.55,t=7.25,P<0.05).The expression levels of active Caspase-3 in the SKOV3 cells in myricetin groups had increasing tendency,and the expression levels of γ-H₂AX in the SKOV3 cells in myricetin groups were higher than that in control group(t=5.41,t=3.21,t=6.58,P<0.05). Conclusion Myricetin can inhibit the proliferation of SKOV3 cells and induce the apoptosis;its possible mechanism may be related to DNA double strands break.

Key words: myricetin, ovarian neoplosms, SKOV3 cells, cell apoptosis, DNA double strands break