Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (05): 914-918.doi: 10.13481/j.1671-587x.20150506

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Inhibitory effects of niflumic acid on apoptosis induced by cobalt chloride in hippocampus neurons

TIAN Jing1, ZOU Rongjun1, LIANG Zufeng1, SHI Wanting1, QI Ling2, LI Yan3   

  1. 1. Department of Physiology, Jilin Medical College, Jilin 132013, China;
    2. Department of Pathology, Jilin Medical College, Jilin 132013, China;
    3. Department of Pathogenic Biology, Jilin Medical College, Jilin 132013, China
  • Received:2015-01-08 Online:2015-09-28 Published:2015-09-29

Abstract:

Objective To explore the inhibitory effects of niflumic acid (NFA) on the apoptosis of hippocampal neurons induced by cobalt chloride (CoCl2),and to elucidate the possible mechanisms. Methods The hippocampal neurons of rats were isolated and cultured,and inverted phase contrast microscope was used to observe the morphological changes of neurons.The hippocampal neurons were cultured and randomly divided into control group (1% serum medium),CoCl2 injury group (200 μmol·L-1 CoCl2) and different concentrations(20,40,80 μmol·L-1)of NFA groups.The survival rates of hippocampal neurons in various groups were measured by MTT assay.The apoptotic rates of hippocampal neurons were analyzed by flow cytometry.ELISA method was performed to observe the expression levels of apoptosis associated proteins Bax,Bcl-2,caspase-3 and hypoxia-inducible factor 1α (HIF-1α) in the supernatant of neurons. Results The hippocampal neurons showed regular shapes and the synapses reticulated after cultured for 7 d. Compared with control group,the survival rate of neurons in CoCl2 injury group was decreased (P<0.01).Compared with CoCl2 injury group,the survival rates of neurons in 20,40,and 80 μmol·L-1 NFA groups were increased significantly (P<0.05 or P<0.01).The results of flow cytometry showed that compared with control group,the apoptotic rate in CoCl2 injury group was increased (P<0.05);compared with CoCl2 injury group,the apoptotic rates of neurons in 20,40,and 80 μmol·L-1 NFA groups were decreased (P<0.05 or P<0.01).The ELISA results showed that the expression levels of HIF-1α,caspase-3,and Bax in the supernatant of neurons in CoCl2 injury group were increased compared with control group(P<0.01);compared with CoCl2 injury group,the expression levels of HIF-1α,caspase-3,and Bax in the supernatant of neurons in 20 and 40 μmol·L-1 NFA groups were decreased,while the expression level of Bcl-2 was increased (P<0.05 or P<0.01). Conclusion NFA can protect the hippocampal neurons from hypoxia injury induced by CoCl2,which may be related to suppressing the expression of HIF-1α,reducing the ratio of Bax/Bcl2 and suppressing the apoptosis of neurons.

Key words: niflumic acid, hippocampal neurons, cobalt chloride, apoptosis

CLC Number: 

  • R737.33