Journal of Jilin University Medicine Edition ›› 2016, Vol. 42 ›› Issue (04): 637-641.doi: 10.13481/j.1671-587x.20160401

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Induction effect of NPPB on apoptosis of human glioma SHG-44 cells and its mechanism

TIAN Jing1, QI Ling2, JI Pengyan3, SHEN Nan3, CUI Wanli1, WANG Chunyan1   

  1. 1. Department of Physiology, Jilin Medical College, Jilin 132013, China;
    2. Department of Pathology, Jilin Medical College, Jilin 132013, China;
    3. Experimental Center, Jilin Medical College, Jilin 132013, China
  • Received:2015-07-07 Online:2016-07-28 Published:2016-07-20

Abstract:

Objective:To investigate the induction effect of NPPB,a chloride channel blocker,on the apoptosis of human glioma SHG-44 cells,and to explore its mechanism. Methods: The SHG-44 cells were cultured in vitro and divided into control group and NPPB groups (50,100,200 μmol·L-1). The cell viability was detected by MTT assay. The apoptotic rates were detected by flow cytometry. The expression levels of Bax,Bcl-2 and caspase-3 were detected by immunohistochemical analysis and Western blottingmethod. Results: Compared with control group,the cell viabilities of SHG-44 cells in 100 and 200 μmol·L-1 NPPB groups after treated for 24 and 48 h were decreased significantly(P < 0.01). The results of flow cytometry showed that the apoptotic rates of SHG-44 cells in 100 and 200 μmol·L-1 NPPB groups were 24.64% and 41.85%,and they were higher than that in control group(4.17%)(P < 0. 01).The immunohistochemical analysis and Western blotting results showed that the expression levels of caspase-3 and Bax proteins in SHG-44 cells in 100 μmol·L-1 NPPB group were increased(P < 0.05 or P < 0. 01),and the expression level of Bcl-2 protein was decreased(P < 0.05). Conclusion:NPPB could induce the apoptosis of human glioma SHG-44 cellsby the down-regulation of the expression of Bcl-2 and the up-regulation of the expression of Bax,and the activation of caspase-3.

Key words: chloride channel, glioma, cell proliferation, apoptosis

CLC Number: 

  • R739.41