硫化氢及其合成酶在膀胱癌细胞株中的表达及其作用

doi:10.13481/j.1671-587x.20160501

Abstract

Abstract:

Objective: To explore the expressions of endogenous hydrogen sulfide (H₂S) and its synthases cystathionine beta synthase (CBS) and cystathionine gamma lyase (CSE) in the cell lines of normal bladder and bladder cancer, and to clarify their mechanism in the development of bladder cancer. Methods: The bladder cancer cell lines (5637, T24, UM-UC-3, EJ) and human bladder epithelial cell line SV-HUC-1 were selected. The expressions of CBS and CSE in bladder cancer and normal cell lines were analyzed by Western blotting assay and the productivities of H₂S in cell lines were detected by sensitive sulphur electrode assay. The EJ cells were selected based on the previous experimental results and divided into groups as follows:① 10 μmol·L^-1 NaHS group, 50μmol·L^-1 NaHS group, 100μmol·L^-1 NaHS group and control group. After drug treatment, the cell survival rate was measured by MTT assay at 24 and 48 h. ② 5 μg·L^-1 cisplatin group, cisplatin (5 μg·L^-1)+NaHS (100 μmol·L^-1) group and control group. After medicine treatment, the cell survival rate was measured by MTT assay and the cell apoptotic rate was detected by flow cytometry at 48 h. Results: Compared with the normal bladder cells (SV-HUC-1), the expression levels of CBS and CSE and the productivity of H₂S in the bladder cancer cell lines (5637, T24,UM-UC-3 and EJ) were increased obviously (P<0.05or P<0.01). Compared with control group, exogenous H₂S promoted the cell proliferation of EJ cells. The cell survival rates were increased with the increase of drug dose (P<0.05), which showed a dose-dependent effect. The cell survival rates were increased with the prolongation of time(P<0.05), which showed a time-dependent effect. After medicine treatment, compared with cisplatin group, the cell viability in cisplatin+NaHS group was increased (P<0.05) and the apoptotic rate was decreased (P<0.05). Conclusion: Endogenous H₂S and its synthases CBS and CSE have an increased expression level in bladder cancer cell lines compared with the normal bladder cells. H₂S can enhance the proliferation of bladder cancer cells and decrease the apoptosis induced by cisplatin.

Key words: hydrogen sulfide, cystathionine beta synthase, cystathionine gamma lyase, bladder neoplasms

CLC Number:

R737.14

SONG Zhiqiang, SHEN Haishan, WANG Wenjia, WU Jianchen, LI Ranwei, LI Shengwen. Expressions of hydrogen sulfide and its synthases in bladder cancer cell lines and their effects[J].Journal of Jilin University Medicine Edition, 2016, 42(05): 843-847.

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Expressions of hydrogen sulfide and its synthases in bladder cancer cell lines and their effects

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