纤维型肌动蛋白对间充质干细胞衰老的调节作用及其机制

doi:10.13481/j.1671-587x.20180305

Abstract

Abstract: Objective: To investigate the regulation effects of fibrous-actin (F-actin) on senescent of the human bone marrow mesenchymal stem cells (hBMSCs), and to elucidate the molecular mechanisms of senescence the of hBMSCs. Methods: The hBMSCs were cultured in vitro and divided into control group (P2 hBMSCs), F-actin inhibitor group (P2 hBMSCs were treated with 2.5 μmol·L^-1 F-actin inhibitor Latrunculin B for 1 h) and P11 hBMSCs group (P11 hBMSCs passaged from P2 hBMSCs continuously). The hBMSCs in various groups were induced by osteogenic, adipogenic and chondrogenic induction mediums and the induction effects were identified by Alizarin red staining, SO staining and oil red O staining. The number of Ki67 positive cells, polymerization and morphology of F-actin and YAP subcellular localization were detected by immunofluorescence staining. SA-β-Gal staining was uesd to detect the SA-β-Gal staining positive cells in the hBMSCs in various groups. Results: The Alizarin red staining, SO staining and oil red O staining results showed that the hBMSCs in various groups had the osteogenic, adipogenic and chondrogenic abilities.The immunofluorescence staining and SA-β-Gal staining results showed that the microfilament bundles in the hBMSCs in control group were more thick, the F-actin length was longer, and the YAP mainly concentrated in the nucleus (YAP was activated in the nucleus). Compared with control group, the number of Ki67 positive cells in P11 hBMSCs group was less and the number of SA-β-Gal positive cells was more;the Factin was shorter and thinner,the YAP mainly concentrated in the cytoplasm,and the high cytoplasmic YAP cells was positive for SA-β-Gal staining;in F-actin inhibitor group,the high cytoplasmic YAP inactivation was found in the hBMSCs and the SA-β-Gal staining positive hBMSCs had more senescent cells. Conclusion: To inhibit the entering of YAP into the nucleus can promote the senescence of hBMSCs and F-actin can affect the senescence of hBMSCs by regulating the YAP activity.

Key words: senescence, mesenchymal stem cells, fibrous-actin YAP protein

CLC Number:

R329.2

YU Dongsheng, CAI Damin, CHEN Jieyu, LYU Fangyi, HUA Kouzhen, YIN Guolin, WANG Junjuan. Regulation effect of F-actin on senescence of mesenchymal stem cells and its mechanism[J].Journal of Jilin University Medicine Edition, 2018, 44(03): 483-486.

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Regulation effect of F-actin on senescence of mesenchymal stem cells and its mechanism

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