寡核苷酸YW002对人牙周膜干细胞增殖、周期、凋亡和早期成骨分化的影响

doi:10.13481/j.1671-587x.20190211

Abstract

Abstract: Objective: To investigate the effects of oligodeoxynucleotide(ODN) YW002 on the proliferation, cell cycle, apoptosis and early osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs), and to clarify the regulation effect of ODN YW002 on the biological properties of hPDLSCs.Methods: The HPDLSCs were cultured until the third to the fifth generations. The cells were divided into PBS blank control group, ODN MT01 group and ODN YW002 group and incubated for 1, 2, 3 and 5 d. The proliferation activities of the hPDLSCs in various groups were detected by MTT assay. The cell cycle and apoptosis of the hPDLSCs in various groups were detected by flow cytomety.The alkaline phosphates(ALP) activities of the hPDLSCs in various groups were detected by alkaline phosphatase colorimetric assay.Results: Compared with blank control group, the proliferation activities of hPDLSCs in ODN YW002 group at 1 and 3 d after culture were increased (P<0.01); the proliferation activity was inceased at 5 d after culture, but there was no significant difference(P>0.05). Compared with ODN MT01 group, the proliferation activity of hPDLSCs in ODN YW002 group at 1 d after culture was decreased (P<0.05); the proliferation activities were increased at 3 and 5 d after culture, but there were no significant differences(P>0.05). Compared with blank control and ODN MT01 group, the percentage of hPDLSCs in G₀/G₁ phase in ODN YW002 group was decreased at 1 d after cultrue,but there was no significant difference(P>0.05); the percentage of hPDLSCs in S phase was increased at 1 d after culture,but there was no significant difference (P>0.05). Compared with blank control group, the early and late apoptotic rates of hPDLSCs in ODN YW002 group were decreased at 1 d after culture (P<0.05);the early and late apoptotic rates were decreased at 2 d after culture, but there were no significant differences(P>0.05).Compared with ODN MT01 group, the early and late apoptotic rates of hPDLSCs in ODN YW002 group were decreased at 2 d after culture (P<0.05). Compared with blank control group, the ALP activities of hPDLSCs in ODN YW002 group were increased at 1, 3 and 5 d after culture(P<0.01).Compared with ODN MT01 group, the ALP activities of hPDLSCs in ODN YW002 group were increased at 1 and 5 d after culture(P<0.05), but the ALP activity was decreased at 3 d after culture (P<0.05).Conclusion: ODN YW002 can promote the proliferation of hPDLSCs by inhibiting the apoptosis, and increase the ALP activity, suggesting that ODN YW002 has the function of promoting the osteogenic differentiation of hPDLSCs.

Key words: oligodeoxynucleotide, periodontal ligament stem cells, cell proliferation, osteogenic differentiation, apoptosis

CLC Number:

R781

BI Xueting, SHEN Yuqin, XU Xiaowei, LI Wenjie, WANG Zhuoran, LIN Chongtao. Effects of oligodeoxynucleotide YW002 on proliferation, cell cycle, apoptosis and early osteogenic differentiation of human periodontal ligament stem cells[J].Journal of Jilin University(Medicine Edition), 2019, 45(02): 273-279.

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Effects of oligodeoxynucleotide YW002 on proliferation, cell cycle, apoptosis and early osteogenic differentiation of human periodontal ligament stem cells

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