Abstract: Objective:To analyze the results of bacteria distribution of wound secretion and the drug susceptibility test of the infected patients from Department of Hand Surgery,and to provide the evidence for antibiotic selection in the infected patients in early stage of treatment. Methods:The clincal data of hospitalized patients from Department of Hand Surgery were collected.The patients were divided into three groups according to the wound conditions. The patients in Group 1 who had no wound while being in hospital were infected after operation;the patients in Group 2 had the contaminated wound but no infection while being in hospital;the patients in Group 3 had definite infection while being in hospital. The results of bacterium culture of wound secretion and drug susceptibility test of the patients in three groups were analyzed. Results:The clinical data of 297 patients were obtained, including 17 patients from Group 1, 201 patients from Group 2, and 79 patients from Group 3. A total of 406 strains of 54 species of bacteria were isolated from 297 patients,including 178 strains(43.84%,178/406) gram-positive bacteria and 228 strains(56.2%,228/406) gram-negative bacteria. The four most common strains were Staphylococcus aureus(15.8%,64/406), Staphylococcus epidermidis(14.0%,57/406),Enterobacter cloacae(10.3%,42/406),and Serratia marcescens(9.9%,40/406).The results of drug susceptibility test showed that the detection rates of methicillin-resistant staphylococcus aureus(MRSA) and staphylococcus epidermidis were 9.4%(6/64) and 72.0%(46/64), respectively. There were no vancomycin-resistant, linezolid-resistant, and tigecycline-resistant positive strains among Staphylococcus susceptibility;and there were no the carbapenem-resistant positive strains among Enterobacter cloacae and Serratia marcescens.The susceptibility rate of Enterobacter cloacae to ceftriaxone was 83.3%(35/42), and its susceptibility to ceftazidime was 90.5%(38/42). The susceptibility rate of Serratia marcescens to ceftriaxone was 100.0%(40/40), and its susceptibility to ceftazidime was 97.5%(39/40). Conclusion:The first generation of cephalosporin and penicillinase-stabilized penicillin can be used as the preferred empirical antibiotics for the infected patients from Deparment of Hand Surgery in our hospital.The third and fourth generation cephalosporins and quinolones antibiotics can be used as the preferred antibiotics for the infected patients with open trauma as well as the possibility of G-bacillus infection.

Key words: infection, bacterial culture, drug susceptibility test, window period, antibiotic, empirical treatment