M1型巨噬细胞相关因子在重度慢性牙周炎患者牙龈组织中的表达及其意义

doi:10.13481/j.1671-587x.20200227

Abstract

Abstract: Objective: To investigate the expressions of M1 macrophage-related factors induced nitric oxide synthase(iNOS) and signal transducers and activators of transcription 1(STAT1) in gingiva tissue of the patients with severe chronic periodontitis, and to elucidate the possible role of M1 macrophages in the occurrence and development of chronic periodontitis. Methods: The gingiva tissue of 15 patients with severe chronic periodontitis was selected as experimental group, and 15 cases of healthy gingiva tissue of the patients requiring removal of the third molars were used as control group.The expression levels of iNOS and STAT1 mRNA in the gingiva tissues of the patients in two groups were detected by RT-PCR.The expression levels of iNOS and STAT1 proteins in the gingiva tissues of the patients in two groups were detected by Western blotting method. Results: Compared with control group,the expression levels of iNOS and STAT1 mRNA in the gingiva tissue of the patients in experimental group were significantly increased (P<0.01);compared with control group,the expression levels of iNOS and STAT1 proteins in the gingiva tissue of the patients in experiment group were also significantly increased (P<0.01). Conclusion: M1 macrophage-mediated immune response is enhanced in severe chronic periodontitis, suggesting that M1 macrophages are involved in the inflammatory response and tissue damage of periodontal tissue.

Key words: M1 macrophage, chronic periodontitis, induced nitric oxide synthase, signal transducers and activators of transcription 1

CLC Number:

R781.42

CAI Rujia, GUAN Ning, LIU Yizhen, GAO Xiuqiu, WANG Linyuan. Expressions of M1 macrophage-related factors in gingiva tissue of patients with severe chronic periodontitis and their significances[J].Journal of Jilin University(Medicine Edition), 2020, 46(02): 372-376.

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Expressions of M1 macrophage-related factors in gingiva tissue of patients with severe chronic periodontitis and their significances

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