Journal of Jilin University Medicine Edition

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Application  of phage-display peptide library in screening short peptides binding  specifically to high metastatic potential hepa

HUANG Wei-li1,3,LYU Yong-chen2,CHI Bao-rong3   

  1. 1.Department of Gastroenterology,Affiliated Hospital,Beihua University,Jilin 132011,China;2.Department of Gastroenterology,First Hospital,Jilin University,Changchun 130021,China;3. Department of General Surgery,Central Hospital of Jilin City,Jilin Province,Jilin 132011,China
  • Received:2013-08-11 Online:2014-01-28 Published:2014-01-25

Abstract:

Abstract:Objective  To explore the application of c7c phage-display peptide library in screening the short peptides which bind specifically to high metastatic potential hepatocellular carcinoma cells HCCLM3,and to lay foundation for research on the molecular mechanism of hepatoma metastasis and detection of the markers of hepatoma metastasis.Methods The human high metastatic potential hepatocellular carcinoma cells HCCLM3 were used as the target cells and the human low metastatic potential hepatocellular carcinoma cells SMMC7721 as the absorber cells for subtraction biopanning from c7c phage-display peptide library.The affinity and specificity of the positive phage clones were identified by ELISA and immunocytochemistry,and the amino acid sequences were deduced by DNA sequencing.Results  After 4 rounds of screening,the phages which can bind to the HCCLM3 cells were enriched obviously (P<0.05). 6 positive phage clones (C4,C6,C7,C10,C11 and C17) with high affinity were identified by ELISA. After cell immunocytochemistry,1 peptide namely C7 with higher specificity to HCCLM3 cells compared with negative control was found (P<0.05) and no conserved motif was found in these peptides.Conclusion The peptides specifically binding to HCCLM3 cells are screened and identified from c7c phage-display peptide library.

Key words: c7c phage-display peptide library, hepatocellular carcinoma, metastasis, short peptide

CLC Number: 

  • R737.25