Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (01): 1-5.doi: 10.13481/j.1671-587x.20150101

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Effects of Akt combined with ionizing radiation onapoptosis, autophagy and proliferation in breast cancer MCF-7 cells

QI Yali1,2, ZHAO Dali3, LIU Yanjun4, GONG Shouliang2, WANG Zhicheng2   

  1. 1. Department of Epidemiology, School of Public Health, Beihua University, Jilin 132001, China;
    2. Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China;
    3. Jilin Province (City) Entry and Exit Inspection and Quarantine Bureau, Jilin 132011, China;
    4. Department of Radiotherapy, People's Hospital of Changchun City, Changchun 130061, China
  • Received:2014-08-27 Online:2015-01-28 Published:2015-01-30

Abstract:

Objective To measure the effects of Akt combined with ionizing radiation on the apoptosis, autophagy and proliferation in the breast cancer MCF-7 cells, and to explore the role of radiotherapy of breast cancer using Akt as a target. Methods The Akt protein expressions in MCF-7, Akt-MCF-7 (Akt over-expression) and Akt-RNAi-MCF-7 (Akt low-expression) cells were detected by Western blotting method after irradiated by 4 Gy X-rays, the apoptotic rates were measured by FCM with AnnexinⅤ-FITC and PI staining, the autopahgic cell percentages were measured by fluorescence microscope with MDC staining, and the cell proliferation activities were detected by MTT assay. Results Compared with MCF-7 cells, the Akt protein expression in Akt-MCF-7 cells was increased, but it was decreased in Akt-RNAi-MCF-7 cells. Compared with MCF-7 group, the apoptotic rates and autophagic percentages in MCF-7+4 Gy, Akt-MCF-7+4 Gy and Akt-RNAi-MCF-7+4 Gy groups were increased significantly (P<0.05 or P<0.01), especially in Akt-RNAi-MCF-7+4 Gy group; compared with MCF-7+4 Gy group, the apoptotic rate and autophagic percentage in Akt-MCF-7+4 Gy group were decreased significantly (P<0.05 or P<0.01), but they were increased significantly in Akt-RNAi-MCF-7+4 Gy group (P<0.05 or P<0.01).Compared with MCF-7 group, the cell proliferation activities in MCF-7+4 Gy group, Akt-MCF-7+4 Gy group and Akt-RNAi-MCF-7+4 Gy group were significantly decreased at different time points(P<0.05 or P<0.01), especially in Akt-RNAi-MCF-7+4 Gy group.Compared decreased with MCF-7+4 Gy group, the cell proliferation activity in Akt-MCF-7+4 Gy group was significantly increased at 24 h (P<0.05), while the cell proliferation activities in Akt-RNAi-MCF-7+4 Gy group were significantly decreased at different time points (P<0.01). Conclusion Akt over-expression can significantly reduce the apoptosis, autophagy and proliferation in MCF-7 cells induced by ionizing radiation, but Akt low-expression has opposite role.

Key words: autophagy, aopotosis, ionizing radiation, breast neoplasms, cell proliferation

CLC Number: 

  • R730.55