外源性表达VEGF165b对人膀胱癌T24细胞侵袭力的影响

doi:10.13481/j.1671-587x.20150113

Abstract

Abstract:

Objective To observe the effects of exogenous expression of VEGF165b on the viability, migrationrate and invasion ability of human bladder cancer T24 cells, and to explore the infulence of VEGF165b in the bilogical features of human bladder cancer cells. Methods The VEGF165b expression vector pcNDA-VEGF165b was constructed.The T24 cells were divided into T24 cells group (control), pcDNA3.0 group (transfected by pcDNA3.0 plasmid) and pcDNA-VEGF165b group (transfected by pcDNA-VEGF165b plasmid).The viability of T24 cells was detected by MTT assay;Western blotting method was used to determine the expressions of VEGF165b, matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9) proteins.Transwell assay was used to detect the rate of cell migration and invasive ability. Results Compared with T24 cells group, the expression levels of VEGF165b, MMP-2 and MMP-9 proteins in the T24 cells in pcDNA3.0 grouphad no significant differences (P>0.05). Compared with T24 cells group or pcDNA3.0 group, the expression level of VEGF165b protein in the T24 cells in pcDNA-VEGF165b group was significantly increased(P<0.05), and the expression levels of MMP-2 and MMP-9 proteins were significantly decreased(P<0.05).The cell viabilities of T24 cells had no significant difference between three groups (P>0.05).Compared with T24 cells group (migration rate 100%), the migration rate of the T24 cells in pcDNA3.0 group (97.2%±7.8%) had no significant difference(P>0.05), but the migration rate in pcDNA-VEGF165b group was decreased significantly (63.5%±10.4%)(P<0.05). Compared with T24 cells group (70.8±8.5), the number of transferred T24 cells in pcDNA3.0 group (66.3±11.2) had no significant difference(P>0.05), but in pcDNA-VEGF165b group (23.5±7.3) it was decreased significantly (P<0.05). Conclusion Exogenous expression of VEGF165b in the T24 cells has no significant effect on the proliferation of T24 cells, but it can reduce the migration and invasion abilities significantly.

Key words: bladder neoplsms, vascular endothelial growth factor 165b, tumor invasion

CLC Number:

R737.14

YIN Jian, ZHANG Siqi, FENG Shuqiang, ZHANG Jin, LI Ranwei. Effect of exogenous expression of VEGF165b on invasion ability of human bladder cancer T24 Cells[J].Journal of Jilin University Medicine Edition, 2015, 41(01): 64-68.

References

[1] Henriquez-Hernandez LA,Navarro P,Luzardo OP,et al.Polymorphisms of glutathione S-transferase mu and theta,MDR1 and VEGF genes as risk factors of bladder cancer: a case-control study[J].Urol Oncol,2012,30(5):660-665.

[2] Biselli-Chicote PM,Oliveira AR,Pavarino EC,et al.VEGF gene alternative splicing: pro- and anti-angiogenic isoforms in cancer[J].J Cancer Res Clin Oncol,2012,138(3):363-370.

[3] Bates DO,Cui TG,Doughty JM,et al.VEGF165b,an inhibitory splice variant of vascular endothelial growth factor,is down-regulated in renal cell carcinoma[J].Cancer Res,2002,62(14):4123-4131.

[4] Josep T,Geoffrey IS,Patricia ML,et al.First-in-humans trail of an RNA interference therapeutic targeting VEGF and KSP in cancer patients with liver involvement[J].Cancer Discov,2013,3(4):406-417.

[5] Perrot-Applanat M.VEGF isoforms[J].Cell Adh Migr,2012,6(6):526-527.

[6] Sitohy B,Nagy JA,Dvorak HF.Anti-VEGF/VEGFR therapy for cancer: reassessing the target[J].Cancer Res,2012,72(8):1909-1914.

[7] Woolard J,Wang WY,Bevan HS,et al.VEGF165b,an inhibitory vascular endothelial growth factor splice variant: mechanism of action,in vivo effect on angiogenesis and endogenous protein expression[J].Cancer Res,2004,64(21):7822-7835.

[8] Rennel E,Waine E,Guan H,et al.The endogenous anti-angiogenic VEGF isoform,VEGF165b inhibits human tumour growth in mice[J].Br J Cancer,2008,98(7):1250-1257.

[9] 任明,梁新,刘禄成,等.荷瘤裸鼠膀胱癌原位模型的建立[J].中国老年学杂志,2007,27(9):827-828.

[10] 孙亚欣,张志超,孙立群,等.基质金属蛋白酶含量及表达与膀胱癌侵袭转移的关系[J].吉林大学学报:医学版,2005,31(1): 127-129.

[11] Merdad A,Karim S,Schulten HJ,et al.Expression of matrix metalloproteinases (MMPs) in primary human breast cancer: MMP-9 as a potential biomarker for cancer invasion and metastasis[J].Anticancer Res,2014,34(3): 1355-1366.

[12] Xu YB,Du QH,Zhang MY,et al.Propofol suppresses proliferation,invasion and angiogenesis by down-regulating ERK-VEGF/MMP-9 signaling in Eca-109 esophageal squamous cell carcinoma cells[J].Eur Rev Med Pharmacol Sci,2013,17(18): 2486-2494.

[13] Li X,Yang Z,Song W,et al.Overexpression of Bmi-1 contributes to the invasion and metastasis of hepatocellular carcinoma by increasing the expression of matrix metalloproteinase MMP-2,MMP-9 and vascular endothelial growth factor via the PTEN/PI3K/Akt pathway[J].Int J Oncol,2013,43(3): 793-802.

[14] Zheng H,Takahashi H,Murai Y,et al.Expressions of MMP-2,MMP-9 and VEGF are closely linked to growth,invasion,metastasis and angiogenesis of gastric carcinoma[J].Anticancer Res,2006,26(5A): 3579-3583.

[15] Hollborn M,Stathopoulos C,Steffen A,et al.Positive feedback regulation between MMP-9 and VEGF in human RPE cells[J].Invest Ophthalmol Visual Sci,2007,48(9): 4360-4367.

Effect of exogenous expression of VEGF165b on invasion ability of human bladder cancer T24 Cells

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Metrics

Comments

Recommended 0

[1]	ZHAO Yanying, WANG Xiuyan, QIN Guotao, SUN Yuanjie, LIU Zhizhong, LI Ranwei. Effect of VEGF165b on biological characteristics of human hepatocellular carcinoma HepG₂ cells [J]. Journal of Jilin University Medicine Edition, 2018, 44(01): 101-105.
[2]	SHI Hai-hong，LIN Jian-qing，GUO Qi-xiang， WU Xin-quan，YU Yi-huang，CHEN Xiang-rong. Expression of TRPC6 in human breast cancer cells and its influence in invasion potential of breast cancer cells [J]. Journal of Jilin University Medicine Edition, 2014, 40(06): 1221-1225.
[3]	SU Jing,ZHOU Lei,ZHANG Hong-yu,SUN Lian-kun,KANG Jin-song. Effect of ClC-2 antisense oligonucleotide on invasion suppression of human U251 cells by cisplatin [J]. J4, 2009, 35(1): 43-46.