miR-18a对结直肠癌SW116细胞辐射敏感性的影响

doi:10.13481/j.1671-587x.20150207

Abstract

Abstract:

Objective To discuss the relationship between miR-18a and the irradiation sensitivity of the colorectal cancer SW116 cells,and to elucidate the possible mechanism of the effects of miR-18a on the apoptosis and autophagy of the cells.Methods The SW116 cells were divided into miR-18a NC group,miR-18a mimicgroup, miR-18a NC+4Gy group,and miR-18a mimic+4Gy group. The expression of miR-18a in SW116 cells was detected by qRT-PCR before and after irradition;colony-forming assay was used to detect the effect of miR-18a on the irradiation sensitivity of the SW116 cells;GFPLC3 morphological assay was used to detect the autophagy rate of SW116 cells;the apoptotic rate of SW116 cells was analyzed by flow cytometry;the target gene of miR-18a was predicted by bioinformatics methods and dual-luciferase reporter assay was used to demonstrate the binding of miR-18a and 3'UTR targets;the expression of ataxia-telangiectasia mutated(ATM) protein in SW116 cells was measured by Western blotting method.Results compared with before irradation,the expression level of miR-18a in the SW116 cells after irradation was decreased obviously (P<0.05). The colony-forming assay results showed that the irradiation sensitivity of the SW116 cells in miR-18a mimic group was increased compared with miR-18a NC group(P<0.05),and the apoptotic rate and autophagy rate of the SW116 cells in miR-18a mimic group were increased (P<0.05). Compared with miR-18a NC+4Gy group,the expression level of ATM protein in miR-18a mimic+4Gy group was decreased.Conclusion ATM is the target gene of miR-18a in the SW116 cells. miR-18a mimic can increase the apoptosis and autophagy induced by irradition,and can increase the radiosensitivity of colorectal cancer SW116 cells.

Key words: miR-18a, colorectal neoplasms, radiation sensitivity, apoptosis, autophagy

CLC Number:

R735.35

YAN Mengmeng, XU Shan, GAO Yan, LIU Yang, HE Mengzi, CHEN Silin, LI Pengwu, LIU Xiaodong, MA Shumei. Effect of miR-18a on irradiation sensitivity of colorectal cancer SW116 cells[J].Journal of Jilin University Medicine Edition, 2015, 41(02): 240-244.

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Effect of miR-18a on irradiation sensitivity of colorectal cancer SW116 cells

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