Abstract:

Objective To investigate the protective effect of Danshenlinzhi(DSLZ) on the cerebral ischemia reperfusion injury in the rats, and to explore its mechanism.Methods 60 Wistar rats were randomly divided into sham group,model group,TanⅡA group, and low,middle,high, doses (5.0,10.0,20.0 mg·kg^-1) of DSLZ groups(n=10). The thread embolism was performed to establish the rat models of focal cerebral ischemia reperfusion injury. The neurologic scores of the rats after 2 h ischemia and 22 h reperfusion were measured and the percentages of the cerebral infarction area of the rats were detected by TTC staining method; the pathomorphology of the brain tissue of the rats was detected by HE staining. The activities of superoxide dismutase (SOD) and levels of malondialdehyde(MDA),nitric oxide (NO) in the brain tissue of the rats were detected.Results Compared with sham group,the neurologic score of the rats in model group was increased(P<0.05),the percentages of the cerebral infarction area was increased(P<0.05),the activity of SOD was decreased(P<0.01) and the levels of MDA and NO were increased (P<0.01). Compared with model group,the neurologic scores of the rats in middle and high doses of DSLZ groups were decreased and the percentages of the cerebral infarction area were decreased,the activities of SOD were increased and the levels of MDA and NO were decreased(P<0.05 or P<0.01).The HE staining results showed the membrane of neurocyte was unclear,and the cells became swelling,deformation or necrosis,severe edema in interstitium and the cell space was enlarged in model group;the nucleolus could be found clearly and there was slight edema in interstitium of the cells of the rats in high dose of DSLZ group.Conclusion DSLZ has a protective effect on the cerebral ischemia reperfusion injury of the rats,and its mechanism may be related to the anti-radical damage and lessening the neurotoxicity of NO.

Key words: Danshenlinzhi, cerebral ischemia reperfusion injury, oxidative stress, superoxide dismutase, malondialdehyde