Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (04): 732-736.doi: 10.13481/j.1671-587x.20150412

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Effects of SiO2 nanoparticles on cell adhesion and migration of HL-7702 cells

PAN Tao1,2, GUO Caixia3, JIN Minghua1, LIU Xiaomei1, LIU Ying1, DU Haiying1, SUN Zhiwei1,4   

  1. 1. Department of Health Toxicology, School of Public Health, Jilin University, Changchun 130021, China;
    2. Department of Public Health, Xi'an Medical University, Xi'an 710021, China;
    3. Department of Occupational and Environmental Health, School of Public Health, Capital Medical University, Beijing 100069, China;
    4. Department of Sanitary Chemistry and Toxicology, School of Public Health, Capital Medical University, Beijing 100069, China
  • Received:2014-10-06 Published:2015-08-01

Abstract:

Objective To study the effects of SiO2 nanoparticles (SiO2 NPs) on the cell adhesion and migration of HL-7702 cells, and to provide experimental basis for safety evaluation of SiO2 NPs. Methods The in vitro cultured human normal liver cell line HL-7702 were randomly divided into control and SiO2 NPs exposure groups at the concentrations of 12.5,25.0,50.0,and 100.0 mg·L-1,respectively.Transmission electron microscope (TEM) and dynamic light scattering (DLS) method were used to observe the characteristics of SiO2 NPs.After 24 h exposure,cell adhesion assay was applied to examine the changes of cell adherence;wound healing assay was used to discover the changes of cell migration;the cellular uptake and biodistribution of SiO2 NPs were observed using TEM. Results Based on the result of TEM,SiO2 NPs were spherically shaped,uniformly sized and sporadically dispersed with the diameter of (67.42±5.69) nm. The results of DLS method showed that the average hydrated particle size of SiO2 NPs dispersing in RPMI-1640 serum-free medium was (134.13±2.78) nm,while the hydrated particle sizes of SiO2 NPs dispersing in RPMI-1640 with 1%,5%,or 10% serum were apparently enlarged.The results of cell adhesion and wound healing assays showed that the adhesion rates and the healing rates of cells could be down-regulated by SiO2 NPs even under a dose that did not affect the cell viability and membrane integrity(P<0.05),furthermore,the down regulation effect was significantiy increased along with the enlargement of the SiO2 NPs' concentration. The TEM results showed that after exposed to nontoxic dose of SiO2 NPs,the cells accumulated a large number of nanoparticles,the particles mostly dispersed in endocytic recycling compartment,and some were in the cytoplasm;some parts of the cell membrans were caved,and the procedure of endocytose could be captured. Conclusion SiO2 NPs could inhibit the cell adhesion and migration in a dose-dependent manner.

Key words: SiO2 nanoparticles, HL-7702 cells, cell adhesion, cell migration, cellular uptake

CLC Number: 

  • R992