Abstract:

Objective To observe the effect of Xuebijing on the expression of cytochrome C(Cyt C) protein and apoptosis of neurons in the rats with ischemia reperfusion injury,and to explore the mechanism. Methods 108 Waster rats were randomly divided into sham operation group,model group,nimodipine group,and Xuebijing group (n=27).The rat models of cerebral ischemia reperfusion were established by middle cerebral artery occlusion for 3 h with suture method.The apoptosis of neurons in perifocal cortex was detected by TUNEL method and the relative expression level of Cyt C protein was analyzed by Western blotting method at 6,12 and 24 h. Results The FUNEL results showed that compared with model group,the number of apoptotic neurons in nimodipine group and Xuebijing group was decreased obviously(P<0.01) at each time point, and there was significant difference between Xuebijing group and nimodipine group at 24 h(P<0.05).The Western blotting results showed that compared with sham operation group,the relative expression levels of Cyt C of the rats in model group were increased obviously at 6,12 and 24 h(P<0.05 or P<0.01). The relative expression level of Cyt C protein of the rats in nimodipine group and Xuebijing group were reduced significantly at every time point compared with model group(P<0.01);the relative expression level of Cyt C protein of the rats in Xuebijing group was lower than that in nimodipine group at 24 h(P<0.01). Conclusion Xuebijing can inhibit the apoptosis after ischemia reperfusion injury,and the mechanism of anti-apoptosis may be related to the down-regulation of the expression of Cyt C protein.

Key words: Xuebijing, cerebral ischemia reperfusion, apoptosis, cytochrome C, rat,Wistar