2011-2013年成都地区发热呼吸道症候群住院患儿鼻病毒流行病学特征

doi:10.13481/j.1671-587x.20160136

Abstract

Abstract:

Objective: To analyze the detection,etiological types and epidemic characteristics of human rhinovirus(HRV)in the hospitalized children with respiratory syndrome in Chengdu area during 2011—2013,and to provide basic data for prevention and control HRV infection.Methods: Altogether 1 117 nasopharyngeal aspirate samples were collected regularly from the children with respiratory syndrome. The samples were screened for the presence of RNA of HRV-5'NCR using RT-PCR.HRV specific sequence types and mutation status were identified by sequencing the VP4/VP2 coding region.Results: A total of 24.62%(275/1 117)of these samples were positive for HRV RNA.30 sequences were obtained,including 15 HRV-As,4 HRV-Bs and 11 HRV-Cs.The HRV detection peak was in November,and in different age groups,the detection rate for children under 1 years of age was higher than others.The highest detection rate of HRV was in the children with asthmatic diseases among different clinical diagnosis diseases.Conclusion: HRV is a common pathogen of viral infection in the hospitalized children with respiratory syndrome and it can be detected throughout the year in Chengdu area.The differences in detection rate between different genders and different seasons are satistically significant.Sequence analysis showed that multiple genotypes of rhinovirus are epidemic in common in Chengdu area and HRV-A is the main subtype.

Key words: rhinovirus, VP4/VP2 gene, respiratory syndrome, reverse transcriptase polymerase chain reaction, genotype

CLC Number:

R373.2

DONG Zefeng, ZHENG Tianli, FAN Xuejia, Pei Xiaofang. Epidemic characteristics of rhinovirus in hospitalized children with respiratory syndrome in Chengdu during 2011-2013[J].Journal of Jilin University Medicine Edition, 2016, 42(01): 177-181.

References

[1] Linsuwanon P,Payungporn S,Suwannakarn K,et al. Complete coding sequence characterization and comparative analysis of the putative novel human rhinovirus (HRV)species C and B[J]. Virol J,2011,8(5):1-12.

[2] Tapparel C,Cordey S,Junier T,et al.Rhinovirus genome variation during chronic upper and lower respiratory tract infections[J].PLoS One,2011,6(6):e21163.

[3] Linder JE,Kraft DC,Mohamed Y,et al.Human rhinovirus C:Age,season,and lower respiratory illness over the past 3 decades[J].J Allergy Clin Immunol,2013,131(1):69-77,e1-6.

[4] Adriana LC,Jaime MR,Rebeca GR,et al.Clinical characteristics and genetic variability of human rhinovirus in Mexico[J].Viruses,2012,4(2):200-210.

[5] Ferguson PE,Gilroy NM,Faux CE,et al.Human rhinovirus C in adult haematopoietic stem cell transplant recipients with respiratory illness[J].J Clin Virol,2013,56(3):339-343.

[6] Pierangeli A,Ciccozzi M,Chiavelli S,et al.Molecular epidemiology and genetic diversity of human rhinovirus affecting hospitalized children in Rome[J].Med Microbiol Immunol,2013,202(4):303-311.

[7] 赵彦杰,陆柔剑,李亚敏,等.重症急性呼吸道感染患儿中人鼻病毒分型检测及流行病学分析[J].生物技术通讯,2015,26(1):95-97.

[8] 刘沁,张兵,谢志萍,等.长沙地区急性下呼吸道感染住院儿童的病毒病原学分析[J].湖南师范大学学报:医学版,2015,12(1):26-31.

[9] Arden KE,Faux CE,O'Neill NT,et al.Molecular characterization and distinguishing features of a novel human rhinovirus (HRV)C,HRVC-QCE,detected in children with fever,cough and wheeze during 2003[J].J Clin Virol,2010,47(3):219-223.

[10] Bizzintino J,Lee WM,Laing IA,et al.Association between human rhinovirus C and severity of acute asthma in children[J].Eur Respir J,2011,37(5):1037-1042.

[11] Wisdom A,Leitch ECM,Gaunt E,et al.Screening respiratory samples for detection of human rhinoviruses (HRVs)and enteroviruses:comprehensive VP4-VP2 typing reveals high incidence and genetic diversity of HRV species C[J].J Clin Microbiol,2009,47(12):3958-3967.

[12] 丁小芳,张兵,钟礼立,等.100例重症社区获得性肺炎住院儿童的病毒病原学分析[J].临床儿科杂志,2012,30(9):857-861.

[13] 赵百慧,沈佳仁,高烨,等.2009-2010年上海市儿童呼吸道病毒病原谱的建立和分析[J].卫生研究,2011,40(5):635-637.

[14] 张爽,毛乃颖,于德山,等.2011年甘肃地区急性呼吸道感染儿童患者中鼻病毒感染规律的研究[J].病毒学报,2013,29(3):273-279.

[15] 谢正德,肖艳,刘春艳,等.儿童急性下呼吸道感染病毒病原学2007-2010年监测[J].中华儿科杂志,2011,49(10):745-749.

[16] 宋明辉,赵林清,钱渊,等.北京地区急性呼吸道感染儿童中人鼻病毒感染状况研究[J].病毒学报,2013,29(2):97-105.

[17] Kiang D,Kalra I,Yagi S,et al.Assay for 5' noncoding region analysis of all human rhinovirus prototype strains[J].J Clin Microbiol,2008,46(11):3736-3745.

Epidemic characteristics of rhinovirus in hospitalized children with respiratory syndrome in Chengdu during 2011-2013

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	WANG Mingrui, ZHAI Xiuyu, WANG Gang, ZHANG Wenlan, GAO Baoshan, ZHOU Honglan, FU Yaowen. Effects of CYP3A5 genetic polymorphisms on metabolism of tacrolimus in patients after kidney transplantation [J]. Journal of Jilin University Medicine Edition, 2018, 44(03): 537-542.
[2]	XING Shaoji, ZHENG Liansheng, WANG Diandong, ZHANG Xuan, CHI Min. Analysis on association between single nucleotide polymorphism of selenoprotein S gene and risk of liver cancer [J]. Journal of Jilin University Medicine Edition, 2018, 44(03): 558-562.
[3]	LIU Yang, CHEN Kelin, LI Xiangyan, LI Qing, LI Jinghua. Detection of human papillomavirus DNA genotypes in different populations and its clinical significance [J]. Journal of Jilin University Medicine Edition, 2015, 41(06): 1211-1214.
[4]	FU Qianxi, ZHOU Li, TANG Min, ZHANG Mingjun, TANG Xiaojun. Analysis on association between polymorphism of apolipoprotein E genotypes and blood lipid levels in male population [J]. Journal of Jilin University Medicine Edition, 2015, 41(04): 808-813.
[5]	SHI Xin,GUAN Xin,TANG Yuan,LYU Le,QIAO Yi-chunLIU Ya-wen,LI Yong,CHENG Yi. Association between SRC-1 gene polymorphism and type 2 diabetes mellitus [J]. Journal of Jilin University Medicine Edition, 2013, 39(6): 1237-1241.
[6]	LIU Ji, JIA Tian-Jun, LIU Xue-Qing, JIA Xiao-Hui, ZHAI Xue-Qiong. Cloning and identification of C-terminal gene encoding human mannose-binding lectin associated serine protein kinase-2 [J]. J4, 2011, 37(1): 51-55.
[7]	SONG Ming-Xu, LI Li-Hua, DING Su-Ning, LIU Zhi-Hui, ZHOU Xi-Ke. Expressions of platelet-derived growth \|factors in primary adenocarcinoma of stomach tissues \|and significance [J]. J4, 2011, 37(1): 97-101.
[8]	ZHANG Xiao-Xia, SUN Xiao-Qi, ZHANG Song-Ling, GAO Yong-Mei, TIAN Hai-Han. Expression of β-catenin in ovarian serous cystadenocarcinoma tissues and its significances [J]. J4, 2010, 36(3): 536-539.
[9]	WU An-Heng, ZHANG Jia-Ying, ZUO Wen-Jing. Association between single nucleotide polymorphism of interferon-gamma gene +874 site and susceptibility to ovarian cancer [J]. J4, 2009, 35(3): 507-510.
[10]	ZHANG Chen-hao,LIU Zhong-hui，LIU Hai-yan,WANG Yi-nan,GE Jing-yan,YU Fang,TAI Gui-xiang. Expressions of activin A and its binding protein follistatin in pancreatic tissues of streptozotocin-induced diabetic mice and their significances [J]. J4, 2009, 35(2): 292-295.
[11]	ZHANG Xiao-xia,FEI Jun-wei,SHU Chang,NI Jin-song,WANG Xin-rui,LI He-lian. Expression of prostasin in ovarian serous cystadenocarinema tissues and its significances [J]. J4, 2009, 35(2): 280-283.
[12]	JI Kun,WANG Bo,LI Xin,YU Hao,ZHANG Ling,HU Jia-di,ZHAO Xue-jian,LI Yang. Expression of cell death regulatory gene GRIM-19 in human renal clear cell carcinoma tissue and its significance [J]. J4, 2009, 35(1): 146-149.
[13]	LI Ran-wei，WANG Ke，YANG Ze-cheng,LIU Yong-hong,LU Jia-yin,WU Dan-hua，ZHAO Yan-ying. Inhibition of siRNA on VEGF expression in PC-3 cell line and cell proliferation [J]. J4, 2008, 34(4): 585-589.
[14]	CHEN Jian-fang,HU Xiao-chun,ZHANG Ying-li. Expressions of matrix metalloproteinase 8 mRNA in apical granuloma and periapical cyst and their significances [J]. J4, 2008, 34(3): 466-468.
[15]	WANG Kai,PIAO Yun-feng,DING Da-yong,FENG Ye. Expression of imprinted gene PEG10 in human gastric adenocarcinoma tissues and significance [J]. J4, 2008, 34(2): 309-312.